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作物杂志,2020, 第6期: 23–29 doi: 10.16035/j.issn.1001-7283.2020.06.004

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

玉米秸秆消化率全基因组关联分析

林淼1(), 张秋芝1, 史利玉1, 刘蓓2, 王红武2, 潘金豹1()   

  1. 1北京农学院,102206,北京
    2中玉金标记（北京）生物技术股份有限公司,102206,北京
  • 收稿日期:2020-04-12 修回日期:2020-07-08 出版日期:2020-12-15 发布日期:2020-12-09
  • 通讯作者: 潘金豹
  • 作者简介:林淼,研究方向是作物遗传育种,E-mail： 18404984376@163.com
  • 基金资助:
    国家重点研发计划：东华北区早熟抗逆耐密适宜机械化玉米新品种培育(2017YFD0101105-2)

Genome-Wide Association Study of Digestibility of Straw in Maize

Lin Miao1(), Zhang Qiuzhi1, Shi Liyu1, Liu Bei2, Wang Hongwu2, Pan Jinbao1()   

  1. 1Beijing University of Agriculture, Beijing 102206, China
    2Zhongyu Golden Marker (Beijing) Biotechnology Incorporated Company, Beijing 102206, China
  • Received:2020-04-12 Revised:2020-07-08 Online:2020-12-15 Published:2020-12-09
  • Contact: Pan Jinbao

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摘要：

48h体外干物质消化率（48h in vitro dry matter digestibility, 48h IVDMD）是衡量青贮玉米品质的重要指标。为了初步探究玉米秸秆消化率的分子遗传机理,以341份玉米自交系为材料,于2018年在沈阳和通辽种植,收获后测定秸秆48h IVDMD。利用全基因组重测序获得的6 276 612个高质量SNPs进行全基因组关联分析,共检测到153个与玉米秸秆消化率显著相关的SNPs位点（P<1.0×10-6）,4个SNPs显著水平在P<1.0×10-8以上;共找到38个秸秆消化率的候选基因,主要涉及细胞生长发育、防御反应和信号转导等生物学功能。

关键词: 玉米, 秸秆, 48h干物质消化率, 全基因组关联分析

Abstract:

The 48h dry matter digestibility (48h IVDMD) is an important index to measure the quality of silage maize. In order to explore the molecular genetic mechanism of digestibility of 48h IVDMD, 341 maize inbred lines were evaluated for 48h IVDMD in 2018 in Shenyang and Tongliao. These inbred lines were subjected to whole genome sequencing, generating a total of 6 276 612 high quality SNPs. GWAS analysis detected 153 SNPs using the threshold of P <1.0×10-6 and 4 SNPs using the threshold of P < 1.0×10-8. Thirty-eight candidate genes were identified based on the GWAS, and the biological functions of these genes were mainly involved in cell growth and development, defense reaction, and signal transduction.

Key words: Maize, Straw, 48h dry matter digestibility, Genome-wide association study

图1

秸秆48h IVDMD频率分布

表1

两个环境秸秆48h IVDMD统计分析

地点
Location		 均值
Mean (%)		 变异范围
Range (%)		 变异系数
Coefficient of variation (%)		 偏度
Skewness (%)		 峰度
Kurtosis (%)		 相关系数
Correlation coefficient		 广义遗传力
Broad-sense heritability (%)
沈阳Shenyang
沈阳Shenyang 80.96 67.24~91.41 4.85 -0.64 1.04
通辽Tongliao 67.94 49.75~85.94 9.50 -0.40 -0.24 0.63** 71.98

图2

不同环境（沈阳和通辽）秸秆48h IVDMD全基因组关联曼哈顿图 实线和虚线分别代表显著阈值8.0和6.0

图3

不同环境秸秆48h IVDMD全基因组关联QQ图 红线表示零假设预期分布,蓝线表示观察到的和通辽秸秆48h IVDMD百分比的关联分布

图4

显著SNPs在染色体上分布

图5

两个环境秸秆48h IVDMD全基因组关联曼哈顿图及其QQ图（MLM）

图6

变异基因的位置

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