Crops ›› 2020, Vol. 36 ›› Issue (4): 107-113.doi: 10.16035/j.issn.1001-7283.2020.04.015

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Genetic Diversity Analysis of Sunflower (Helianthus annuus L.) Based on DUS Testing

Shan Feibiao1,2(), Du Ruixia1,2, Wang Yongxing1,2, Yang Qinfang1,2, Liu Chunhui1,2, Chen Yang1   

  1. 1Bayannur Academy of Agricultural and Animal Sciences, Linhe 015000, Inner Mongolia, China
    2Bayannur Sub-Center for Plant Variety Tests, Ministry of Agriculture and Rural Affairs, Linhe 015013, Inner Mongolia, China
  • Received:2019-12-13 Revised:2020-03-02 Online:2020-08-15 Published:2020-08-11

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Abstract:

Forty-three basic traits in NY/T 2433-2013 were used to evaluate the genetic diversity of 63 sunflower varieties from Bayannur Sub-Center and compared with 32 similar varieties. The results showed that a total of 124 allelic variations were detected in the phenotypic characters traits of the varieties with an average of 3.0244 per trait ranged from 1 to 8. The average shannon’s diversity index (H') of tested varieties was 0.5582, ranged from 0 to 1.8101. Among the 41 valid test traits, almost 17.07% of all the traits had more than 5 allelic variations and 51.22% of all the traits had more than three allelic variations. The genetic diversity of tested varieties was higher than the similar varieties compared to their total alleles, alleles per trait and H'-value. In appearance, the genetic diversity of the tested varieties was more abundant than the similar varieties. The UPGMA clustering analysis showed that 95 sunflower varieties could be divided into two groups when the similarity coefficient was 0.860. The results of principal coordinate analysis were basically consistent with UPGMA cluster analysis but the genetic similarity among 95 sunflower varieties could be judged more vividly by the position of sunflower varieties in the principal coordinate. There was a little differences between the tested varieties and similar varieties. Therefore, it is suggested that breeders should broaden the selection materials of sunflower parents so as to promote the innovation of sunflower materials and the breeding of new varieties.

Key words: DUS testing, Sunflower (Helianthus annuus L.), Diversity index

Table 1

Testing materials"

测试编号
Test number		 测试类型
Test type		 编码
Code		 测试编号
Test number		 测试类型
Test type		 编码
Code		 测试编号
Test number		 测试类型
Test type		 编码
Code
BYNER20170114B 近似品种 JS1 BYNER20170114A 参试品种 SQ001 BYNER20170151A 参试品种 SQ033
BYNER20170116B 近似品种 JS2 BYNER20170115A 参试品种 SQ002 BYNER20170152A 参试品种 SQ034
BYNER20170117B 近似品种 JS3 BYNER20170116A 参试品种 SQ003 BYNER20170153A 参试品种 SQ035
BYNER20170118B 近似品种 JS4 BYNER20170117A 参试品种 SQ004 BYNER20170154A 参试品种 SQ036
BYNER20170119B 近似品种 JS5 BYNER20170118A 参试品种 SQ005 BYNER20170155A 参试品种 SQ037
BYNER20170120B 近似品种 JS6 BYNER20170119A 参试品种 SQ006 BYNER20170156A 参试品种 SQ038
BYNER20170121B 近似品种 JS7 BYNER20170120A 参试品种 SQ007 BYNER20170157A 参试品种 SQ039
BYNER20170122B 近似品种 JS8 BYNER20170121A 参试品种 SQ008 BYNER20170159A 参试品种 SQ040
BYNER20170123B 近似品种 JS9 BYNER20170122A 参试品种 SQ009 BYNER20170160A 参试品种 SQ041
BYNER20170124B 近似品种 JS10 BYNER20170123A 参试品种 SQ010 BYNER20170161A 参试品种 SQ042
BYNER20170125B 近似品种 JS11 BYNER20170124A 参试品种 SQ011 BYNER20170162A 参试品种 SQ043
BYNER20170126B 近似品种 JS12 BYNER20170125A 参试品种 SQ012 BYNER20170163A 参试品种 SQ044
BYNER20170131B 近似品种 JS13 BYNER20170126A 参试品种 SQ013 BYNER20170164A 参试品种 SQ045
BYNER20170132B 近似品种 JS14 BYNER20170127A 参试品种 SQ014 BYNER20170166A 参试品种 SQ046
BYNER20170135B 近似品种 JS15 BYNER20170128A 参试品种 SQ015 BYNER20170167A 参试品种 SQ047
BYNER20170137B 近似品种 JS16 BYNER20170129A 参试品种 SQ016 BYNER20170168A 参试品种 SQ048
BYNER20170142B 近似品种 JS17 BYNER20170130A 参试品种 SQ017 BYNER20170169A 参试品种 SQ049
BYNER20170143B 近似品种 JS18 BYNER20170131A 参试品种 SQ018 BYNER20170170A 参试品种 SQ050
BYNER20170145B 近似品种 JS19 BYNER20170132A 参试品种 SQ019 BYNER20170172A 参试品种 SQ051
BYNER20170147B 近似品种 JS20 BYNER20170135A 参试品种 SQ020 BYNER20170174A 参试品种 SQ052
BYNER20170156B 近似品种 JS21 BYNER20170136A 参试品种 SQ021 BYNER20170175A 参试品种 SQ053
BYNER20170159B 近似品种 JS22 BYNER20170137A 参试品种 SQ022 BYNER20170176A 参试品种 SQ054
BYNER20170167B 近似品种 JS23 BYNER20170138A 参试品种 SQ023 BYNER20170177A 参试品种 SQ055
BYNER20170172B 近似品种 JS24 BYNER20170140A 参试品种 SQ024 BYNER20170178A 参试品种 SQ056
BYNER20170176B 近似品种 JS25 BYNER20170141A 参试品种 SQ025 BYNER20170179A 参试品种 SQ057
BYNER20170177B 近似品种 JS26 BYNER20170142A 参试品种 SQ026 BYNER20170180A 参试品种 SQ058
BYNER20170182B 近似品种 JS27 BYNER20170143A 参试品种 SQ027 BYNER20170181A 参试品种 SQ059
BYNER20170184B 近似品种 JS28 BYNER20170144A 参试品种 SQ028 BYNER20170182A 参试品种 SQ060
BYNER20170187B 近似品种 JS29 BYNER20170145A 参试品种 SQ029 BYNER20170183A 参试品种 SQ061
BYNER20170189B 近似品种 JS30 BYNER20170147A 参试品种 SQ030 BYNER20170184A 参试品种 SQ062
BYNER20170190B 近似品种 JS31 BYNER20170149A 参试品种 SQ031 BYNER20180010A 参试品种 SQ063
BYNER20180010B 近似品种 JS32 BYNER20170150A 参试品种 SQ032

Table 2

Phenotypic traits of sunflower germplasms"

编号Number 性状Trait 类型Type 编号Number 性状Trait 类型Type
chr1 植株：幼茎花青甙显色 QN chr23 叶片：叶尖高度 QN
chr2 *叶片：颜色 QN chr24 叶片：叶柄与主茎夹角 QN
chr3 叶：花青甙显色 QL chr25 管状花：颜色 PQ
chr4 植株：主茎叶数 QN chr26 管状花：柱头花青甙显色 QN
chr5 植株：茎上部刚毛 QN chr27 花粉：颜色 PQ
chr6 *开花期 QN chr28 植株：主盘与最近侧盘的位置 PQ
chr7 舌状花：形状 PQ chr29 *植株：花盘倾斜度 QN
chr8 *舌状花：颜色 PQ chr30 *花盘：形状 PQ
chr9 舌状花：密度 QN chr31 *植株：分枝 QL
chr10 苞叶：密度 QN chr32 *植株：分枝类型 PQ
chr11 苞叶：形状 PQ chr33 *花盘：大小 QN
chr12 苞叶：花青甙显色 QL chr34 *植株：高度 QN
chr13 苞叶：尖长度 QN chr35 瘦果：大小 QN
chr14 *叶片：大小 QN chr36 瘦果:形状 PQ
chr15 叶片：形状 PQ chr37 瘦果:厚度 QN
chr16 *叶片：锯齿 QN chr38 *瘦果：主色 QL
chr17 叶片：锯齿规则性 QL chr39 瘦果：色斑 QL
chr18 叶片：横截面形状 QN chr40 瘦果：条纹 QL
chr19 叶片：叶翼 QL chr41 瘦果：条纹颜色 PQ
chr20 *叶片：叶耳 QN chr42 *瘦果：边缘条纹 QN
chr21 *叶片：泡状程度 QN chr43 *瘦果：边缘间条纹 QN
chr22 *叶片：侧脉角度 PQ

Table 3

Diversity statistics of 43 basic testing traits among 63 tested varieties and 32 similar varieties"

性状
Trait		 参试品种Tested variety 近似品种Similar variety
Na Ne H＇ Na Ne H＇
chr1 8 5.3419 1.8101 5 3.3247 1.3914
chr2 2 1.3243 0.4101 3 1.2104 0.3708
chr3 2 1.9183 0.6717 2 1.6787 0.5941
chr4 4 2.4822 1.0309 3 2.4976 0.9810
chr5 6 2.3009 1.1334 4 1.5950 0.7614
chr6 4 2.7931 1.1566 3 2.0898 0.8724
chr7 2 1.0322 0.0815 2 1.2800 0.3768
chr8 1 1.0000 0 1 1.0000 0
chr9 6 4.0050 1.5287 5 3.9084 1.4721
chr10 6 3.1178 1.3257 3 2.2165 0.8753
chr11 3 1.8643 0.7413 2 1.6787 0.5941
chr12 1 1.0000 0 1 1.0000 0
chr13 4 2.0900 0.8992 3 1.9617 0.7691
chr14 6 4.1387 1.5149 6 4.1290 1.5663
chr15 1 1.0000 0 1 1.0000 0
chr16 1 1.0000 0 2 1.0644 0.1391
chr17 1 1.0000 0 2 1.0644 0.1391
chr18 2 1.0322 0.0815 2 1.0644 0.1391
chr19 2 1.8000 0.6365 2 1.8221 0.6435
chr20 4 2.1078 0.9845 3 1.9922 0.8449
chr21 4 1.3377 0.5078 3 1.2104 0.3708
chr22 3 2.5361 1.0024 3 2.5990 1.0130
chr23 4 2.0857 0.8233 3 2.1070 0.8060
chr24 3 1.0661 0.1628 1 1.0000 0
chr25 2 1.0322 0.0815 1 1.0000 0
chr26 2 1.0322 0.0815 1 1.0000 0
chr27 1 1.0000 0 1 1.0000 0
chr28 0 0 0 0 0 0
chr29 3 1.0661 0.1628 2 1.0644 0.1391
chr30 2 1.1711 0.2772 1 1.0000 0
chr31 1 1.0000 0 2 1.1327 0.2338
chr32 0 0 0 0 0 0
chr33 2 1.7279 0.6122 2 1.8221 0.6435
chr34 6 2.8575 1.3103 7 2.7978 1.4151
chr35 5 1.7174 0.8361 4 2.9942 1.2075
chr36 3 1.6300 0.6260 2 1.5193 0.5253
chr37 2 1.2082 0.3145 3 1.7239 0.6922
chr38 4 1.2149 0.3971 3 1.2104 0.3708
chr39 2 1.0998 0.1914 2 1.1327 0.2338
chr40 1 1.0000 0 1 1.0000 0
chr41 2 1.3644 0.4376 3 1.8893 0.7482
chr42 3 1.2907 0.4285 3 1.7239 0.6922
chr43 3 1.6300 0.6260 3 1.7239 0.6922
平均
Average		 3.0244 1.7663 0.5582 2.5854 1.7129 0.5442

Fig.1

Cluster map of 95 sunflower varieties based on the similarity coefficients"

Fig.2

Two-dimensional principal coordinate analysis of 95 sunflower varieties based on morphological traits"

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