Crops ›› 2022, Vol. 38 ›› Issue (2): 230-236.doi: 10.16035/j.issn.1001-7283.2022.02.032

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Inhibitory Effect of Chlorogenic Acid on Phytophthora nicotiana and Its Control Effect on Tobacco Black Shank Disease

Zhang Yudan1(), Ma Xiaohan1, Li Junling1, Xu Zicheng1, Jia Wei1,*(), Shi Qiuhuan2,*()   

  1. 1Tobacco College, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Luoyang Branch of Henan Tobacco Company, Luoyang 471003, Henan, China
  • Received:2021-10-18 Revised:2021-12-13 Online:2022-04-15 Published:2022-04-24
  • Contact: Jia Wei,Shi Qiuhuan E-mail:519086134@qq.com;jiawei@henau.edu.cn;3582072897@qq.com

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Abstract:

Tobacco black shank (TBS) caused by Phytophthora nicotianae is one of the most devastating diseases in tobacco production. Chlorogenic acid (CGA) is a natural phenolic compound that exhibits several unprecedented bioactivities including antioxidants and antibacterials. Using in vitro culture and foliar application method, we investigated CGA inhibition on P. nicotianae and CGA control efficiency on TBS, respectively. The results showed that, compared with control, 100g/mL CGA significantly inhibited the growth of P. nicotianae significantly, and the colony diameter changed from 7.51cm to 5.95cm. Spraying the leaves with 100g/mL CGA significantly reduced leaf spot size from 4.60cm to 1.20cm and significantly increased the activities of the antioxidant enzymes POD, SOD and CAT and the defense enzyme PPO by 72.6%, 54.6%, 155.8% and 8.2%, respectively. In addition, the content of soluble protein in leaves sprayed with CGA increased significantly from 3.0mg/g to 5.3mg/g, the content of soluble sugars increased significantly from 2.7mg/g to 1.5mg/g, and the content of proline decreased from 192.7mg/g to 150.4μg/g. Therefore, CGA could directly inhibit the growth of P. nicotiana, improve the resistance to TBS.

Key words: Chlorogenic acid, Tobacco black shank, Phytophthora nicotianae, Osmoregulation substances

Table 1

Effects of different concentrations of CGA on radial growth of P. nicotianae"

处理
Treatment		 菌落直径
Colony diameter (cm)		 抑菌率
Bacteriostatic rate (%)
CK 7.51±0.03a –
CGA1 6.90±0.08b 8.2
CGA2 5.95±0.10c 20.8

Fig.1

Effects of different concentrations of CGA on radial growth of P. nicotianae Different lowercase letters in the same column indicate significant difference at P < 0.05"

Fig.2

Effects of CGA2 treatment on morphology of P. nicotianae"

Fig.3

Effects of CGA2 treatment on cell of P. nicotianae"

Fig.4

Infection results of isolated tobacco leaves"

Fig.5

The results of trypan blue staining"

Fig.6

The results of P. nicotianae infection"

Fig.7

Effects of CGA foliar application on the lesion size in tobacco leaves Different lowercase letters indicate significant difference at 0.05 level, the same below"

Fig.8

Effects of CGA on the enzymes activities in tobacco leaves inoculated with P. nicotianae"

Fig.9

Effects of CGA on soluble sugar, soluble protein and proline contents in tobacco leaves inoculated with P. nicotianae"

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