Crops ›› 2023, Vol. 39 ›› Issue (1): 52-57.doi: 10.16035/j.issn.1001-7283.2023.01.008

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Effects of Debranched and Repeated Wet Heat Treatment on Content and Physicochemical Properties of Resistant Starch of Tartary Buckwheat

Fu Ziping1,2(), Fan Yu1, Lai Dili2,3, Zhang Kaixuan2, Zhu Jianfeng4, Li Jiguang5, Zhou Meiliang2(), Wang Junzhen4()   

  1. 1College of Food and Bioengineering, Chengdu University, Chengdu 610000, Sichuan, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3College of Agriculture, Guizhou University, Guiyang 550000, Guizhou, China
    4Agricultural Science Research Institute of Liangshan Yi Autonomous Prefecture, Liangshan 615000, Sichuan, China
    5Crop Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410000, Hunan, China
  • Received:2022-06-21 Revised:2022-09-16 Online:2023-02-15 Published:2023-02-22

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Abstract:

Starch branch chains of Fagopyrum tataricum were removed by pullulanase (PUL), and the effects of PUL dosage and times of wet and heat treatment (HMT) on resistant starch content and physicochemical properties of F.tataricum whole powder were investigated. The results showed that the content of resistant starch of F.tataricum could be increased by PUL, HMT and PUL-HMT, in which the dosage of PUL was 10U/g (PUL10), and the two times treatment (HMT2) had the best effects, the resistant starch content increased from 4.74% to 13.38%. After PUL-HMT, the surface of F.tataricum powder changed from smooth to concave and convex, the crystal structure changed from A type to A+V type, and the relative crystallinity and enthalpy value decreased. Infrared spectroscopy showed that the short-range ordered structure in starch changed slightly. The chemical properties and resistant starch content of F.tataricum powder could be effectively changed by debranching and HMT, and PUL10-HMT2 treatment could get more ideal F.tataricum material with high resistant starch.

Key words: Fagopyrum tataricum, Pullulanase, Wet heat treatment, Resistant starch, Physicochemical properties

Table 1

Resistant starch content of different treatments"

样品
Sample		 普鲁兰酶用量
Pullulanase
dosage (U/g)		 湿热处理次数
Times of wet
heat treatment		 抗性淀粉含量
Resistant starch
content (%)
PUL0-HMT0 0 0 4.74±0.12f
PUL10-HMT0 10 0 11.58±0.27b
PUL20-HMT0 20 0 10.96±0.08c
PUL0-HMT1 0 1 6.48±0.06e
PUL10-HMT1 10 1 12.82±0.12a
PUL20-HMT1 20 1 12.70±0.27a
PUL0-HMT2 0 2 9.34±0.02d
PUL10-HMT2 10 2 13.38±0.35a
PUL20-HMT2 20 2 13.20±0.26a

Fig.1

Electronic scan of natural samples"

Fig.2

Electronic scan of PUL10-HMT2 samples"

Fig.3

X-ray diffraction of samples under different treatment conditions"

Fig.4

FTIR spectra after different treatment conditions"

Table 2

Ratio of peak strength under different treatment conditions"

样品Sample 1047/1022cm-1 1022/995cm-1
天然样品Natural sample (CK) 1.04±0.26a 0.97±0.26a
PUL10-HMT2 1.01±0.08a 0.99±0.16a

Table 3

Thermodynamic parameters after different treatment conditions"

参数Parameter 天然样品Natural sample PUL10-HMT2
T0 (℃) 75.73±0.08b 80.43±0.54a
Tp (℃) 79.83±0.34b 83.76±0.28a
Tc (℃) 83.50±0.32b 85.67±0.07a
?H (J/g) 5.09±0.14a 1.58±0.21a
?T (℃) 7.77±0.38a 5.24±0.59a
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