利用CRISPR/CAS9基因编辑技术创制香型郑稻19新种质
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王付华,薛华政,王亚,王生轩,王越涛,付景,杨文博,白涛,李俊周,尹海庆
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Breeding Fragrant Rice Zhengdao19 Using CRISPR/Cas9 Mediated Gene Editing Technology
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Fuhua Wang,Huazheng Xue,Ya Wang,Shengxuan Wang,Yuetao Wang,Jing Fu,Wenbo Yang,Tao Bai,Junzou Li,Haiqing Yin
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表2 T1和T2代靶位点序列突变分析
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Table 2 Analysis of mutation in target sequences
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T1株系号
No. of
T1 line | T1测序株Sequenced of T1 | T2测序株Sequenced of T2 | 靶点序列
Target sequence | 突变情况
Mutation | 总株数/突变体
Total/Mutant | 纯合/杂合
Homozygote/Heterzygote
in mutants | 总株数/突变体
Total/Mutant | 纯合/杂合
Homozygote/Heterzygote
in mutants | | WT | | | | | CAAGTACCTCCGCGCAATCGCGGCC | | | T1-2 | 3/3 | 0/3 | 6/6 | 6/0 | CAAGTACCTCCGCGC--TCGCGGCC | -2 | | | | | | CAAGTACCTCCGCG-----CGGCC | -5 | | T1-3 | 3/3 | 2/1 | 6/6 | 3/3 | CAAGTACCTCCGCG--------GCC | -8 | | | | | | CAAGTACCTCCGCGCA-TCGCGGCC | -1 | | T1-6 | 5/4 | 2/2 | 5/4 | 2/2 | CAAGTACCTCCGCGCAAaTCGCGGCC | +1 | | T1-7 | 5/5 | 3/2 | 5/5 | 5/0 | CAAGTACCTCCGC------------ | -12 | | | | | | CAAGTACCTCCGCGCAAaTCGCGGCC | +1 | | T1-8 | 3/2 | 0/2 | 3/3 | 3/0 | CAAGTACCTCCGCGCAAacacttgat | +9 | | T1-9 | 3/3 | 3/0 | 3/2 | 2/0 | CAAGTACCTCCGCGCAAaTCGCGGCC | +1 | | 小计Total | 22/20 | 10/10 | 28/26 | 21/5 | | |
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