内蒙古胡麻地方品种资源遗传多样性分析

伊六喜,斯钦巴特尔,高凤云,周宇,贾霄云,陈明哲,张辉,贾海滨

Genetic Diversity of Flax Germplasm Resources in Inner Mongolia

Liuxi Yi,Bateer Siqin,Fengyun Gao,Yu Zhou,Xiaoyun Jia,Mingzhe Chen,Hui Zhang,Haibin Jia

表3 反应体系L₁₆(4)³正交试验设计

Table 3 Orthogonal design L₁₆(4)³ of reaction system

编号 Code	引物量(μL) Primer quantity	2×Taq (μL) Master Mix	DNA模板量(ng) DNA template quantity
1	0.1	7.0	25
2	0.1	9.0	30
3	0.1	12.0	35
4	0.1	15.0	40
5	0.2	7.0	30
6	0.2	9.0	25
7	0.2	12.0	40
8	0.2	15.0	35
9	0.3	7.0	35
10	0.3	9.0	40
11	0.3	12.0	25
12	0.3	15.0	30
13	0.5	7.0	40
14	0.5	9.0	35
15	0.5	12.0	30
16	0.5	15.0	25