基于不同烟草类型基因组重测序的烟草SSR标记的开发和应用

耿歆淇,杨惠娟,秦艳青,杨兴有,赵世民,史宏志

Development and Application of Tobacco SSR Markers Based on Genome Re-Sequencing of Different Tobacco Types

Xinqi Geng,Huijuan Yang,Yanqing Qin,Xingyou Yang,Shimin Zhao,Hongzhi Shi

表2 样本与参考基因组的质量控制匹配结果

Table 2 Sample and reference genome quality control match result

样本 Sample 有效数据 Clean Reads data PCR重复比例(%) PCR duplicate rate 去除PCR重复后数据(×108) Data after PCR duplication were removed 对比参考基因组的数据(×108) Compare reference genome data 参考基因组匹配率(%) Reference genome mapping rate QY96 904 164 146 16.18 7.58 7.56 99.74 WM3H 917 495 832 15.53 7.75 7.74 99.91 LY1306 887 012 964 14.64 7.57 7.57 99.91 WF1H 913 227 010 15.97 7.67 7.66 99.88