基于不同烟草类型基因组重测序的烟草SSR标记的开发和应用

耿歆淇,杨惠娟,秦艳青,杨兴有,赵世民,史宏志

Development and Application of Tobacco SSR Markers Based on Genome Re-Sequencing of Different Tobacco Types

Xinqi Geng,Huijuan Yang,Yanqing Qin,Xingyou Yang,Shimin Zhao,Hongzhi Shi

表4 各测序品种InDeL检测结果

Table 4 Sequencing species InDeL variant results

样本Sample WF1H WM3H LY1306 QY96 K326 BX 位于基因组上游的突变数The number of mutations in the upstream region 32 016 44 902 41 378 50 940 85 775 69 880 位于基因组下游的突变数The number of mutations in the downstream region 9 836 13 438 12 432 15 352 25 873 20 177 位于基因组区间的突变数The number of mutations in the intergenic region 179 646 226 119 212 089 250 865 437 125 295 984 位于5′端非翻译区域的突变数The number of mutations in the 5′ UTR region 1 334 2 029 1 854 2 299 3 488 3 456 位于3′端非翻译区域的突变数The number of mutations in the 3′ UTR region 1 104 1 604 1 405 1 998 2 968 2 241 位于剪切区域的突变数The number of mutations in the splice region 662 812 749 874 1 081 931 位于内含子区域的突变数The number of mutations in the intron region 12 614 16 880 15 448 19 796 32 146 24 199 位于外显子区域的突变数The number of mutations in the exon region 2 059 2 641 2 450 3 045 4 706 3 468 总突变数The total mutations 239 271 308 425 287 805 345 169 593 162 420 336