基于不同烟草类型基因组重测序的烟草SSR标记的开发和应用

耿歆淇,杨惠娟,秦艳青,杨兴有,赵世民,史宏志

Development and Application of Tobacco SSR Markers Based on Genome Re-Sequencing of Different Tobacco Types

Xinqi Geng,Huijuan Yang,Yanqing Qin,Xingyou Yang,Shimin Zhao,Hongzhi Shi

表6 用于检测不同烟草类型的SSR多态性位点的引物

Table 6 SSR polymorphic site primers used to distinguish different types of tobacco

编号No. 序列名称Seq. name 正向引物Forward primer (5′→3′) 反向引物Reverse primer (5′→3′) 1 NW015795276.1 AAAGTGCGTGGAAAAAATC AGCAGCAGAAGAAAAAGTG 2 NW015854676.1 ATCAAATCCATGCTCCCAA GACCCCAAGATACCCCAAC 3 NW015889872.1 CAAAAGCAGCAAAAACACA GAGAAATGAACGGAAAGTC 4 NW015890969.1 CTTAACCACAAAACCCACA TTCGTAACAATTATCACCG 5 NW015930318.1 GTGTTCCTCTGACTTCTCT CCTTAATCTCAAATCTCTC