黑龙江省主栽水稻品种抗稻瘟病基因的分子检测与分析

周弋力,张亚玲,赵宏森,靳学慧

Molecular Detection and Analysis of Rice Blast Resistance Genes in Main Rice Varieties in Heilongjiang Province

Yili Zhou,Yaling Zhang,Hongsen Zhao,Xuehui Jin

表1 PCR引物序列、扩增片段大小

Table 1 Primer sequence and amplified fragment size for PCR

目的基因Target gene 引物名称Primer name 引物序列(5′→3′) Primer sequence (5′→3′) 预期片段大小Expected size (bp) 酶Enzyme Pi-ta Pi-ta F Pi-ta R AGCAGGTTATAAGCTAGGCC CTACCAACAAGTTCATCAAA 1042 - Pi-ta Npi-ta F Npi-ta R AGCAGGTTATAAGCTAGCTAT CTACCAACAAGTTCATCAAA 1042 - Pi-b Pi-b F Pi-b R GAACAATGCCCAAACTTGAG GGGTCCACATGTCAGTGAGC 365 - Pi-b Npi-b F Npi-b R TCGGTGCCTCGGTAGTCAGT GGGAAGCGGATCCTAGGTCT 803 - Pik-m Pik-m1 F Pik-m1 R TGAGCTCAAGGCAAGAGTTGAGGA TGTTCCAGCAACTCGATGAG 174（抗）/213 - Pik-m Pik-m2 F Pik-m2 R CAGTAGCTGTGTCTCAGAACTATG AAGGTACCTCTTTTCGGCCAG 290（抗）/332 - Pi9 Pi9 F Pi9 R GCTGTGCTCCAAATGAGGAT GCGATCTCACATCCTTTGCT 291（抗）/397 - Pii Pii F Pii R TCCAATGCTTCTGAAAGGTAGC TGGAAACATGAACCCATATCCT 355 PvuⅡ Pi-d3 Pi-d3 F Pi-d3 R TACTACTCATGGAAGCTAGTTCTC ACGTCACAAATCATTCGCTC 658 BamHⅠ