农杆菌介导谷子成熟胚遗传转化体系的建立与优化
李颜方,杜艳伟,张正,王高鸿,赵根有,赵晋锋,余爱丽

Establishment and Optimization of Agrobacterium Mediated Transformation System for Mature Embryo of Foxtail Millet
Yanfang Li,Yanwei Du,Zheng Zhang,Gaohong Wang,Genyou Zhao,Jinfeng Zhao,Aili Yu
表1 试验所用的培养基
Table 1 Media used in experiment
培养基Media 培养基组分Medium composition
愈伤组织诱导培养基（A）
Calli inducing medium		 MS培养基+2.0mg/L 2,4-D+0.5mg/L Kinetin+40g/L麦芽糖+35mg/L ZnSO4·7H2O+0.6mg/L CuSO4·5H2O+
8mg/L AgNO3+4g/L植物凝胶,pH 5.8
分化培养基（B）
Differential medium		 MS培养基+2.0mg/L Kinetin+20g/L麦芽糖+7g/L Phytoblend（植物凝胶类似物）,pH 5.8
农杆菌悬浮侵染培养基（C）
Suspending medium		 MS培养基+40g/L麦芽糖+35mg/L ZnSO4·7H2O+0.6mg/L CuSO4·5H2O+100μmol/L乙酰丁香酮（AS）+1g/L泊洛沙姆,pH 5.8
共培养基（D）
Co-cultural medium		 MS培养基+2.0mg/L 2,4-D+0.5mg/L Kinetin+40g/L麦芽糖+35mg/L ZnSO4·7H2O+0.6mg/L CuSO4·5H2O+
100μmol/L AS+1g/L泊洛沙姆+4g/L植物凝胶,pH 5.8
筛选分化培养基（E）
Screening and differentiation medium		 分化培养基+150mg/L特美汀+5mg/L草丁膦,pH 5.8
筛选再生培养基（F）
Screening and regeneration medium		 MS培养基+2.0mg/L 2,4-D+0.5mg/L Kinetin+40g/L麦芽糖+35mg/L ZnSO4·7H2O+0.6mg/L CuSO4·5H2O+
4g/L植物凝胶+150mg/L特美汀+10mg/L草丁膦,pH 5.8
生根培养基（G）
Rooting medium		 1/2 MS培养基+30g/L蔗糖+7g/L Phytoblend,pH 5.7