农杆菌介导谷子成熟胚遗传转化体系的建立与优化

李颜方,杜艳伟,张正,王高鸿,赵根有,赵晋锋,余爱丽

Establishment and Optimization of Agrobacterium Mediated Transformation System for Mature Embryo of Foxtail Millet

Yanfang Li,Yanwei Du,Zheng Zhang,Gaohong Wang,Genyou Zhao,Jinfeng Zhao,Aili Yu

表2 适宜组织培养的谷子基因型

Table 2 Genotype of millet suitable for tissue culture %

材料编号 Material code 基因型 Genotype 发芽率 Rate of germination 愈伤组织诱导率 Rate of callus induction 抗性愈伤组织率 Rate of resistance callus gus表达率 Rate of gusexpression 19 豫谷1号Yugu 1 83.5 84.2 32.6 11.4 50 长农35 Changnong 35 80.5 83.1 35.8 25.3 63 长生06 Changsheng 06 68.5 85.3 18.9 7.5 125 长生07 Changsheng 07 80.5 81.7 24.6 19.2 129 沁州黄Qinzhouhuang 96.6 87.2 12.6 3.9 164 冀谷11 Jigu 11 78.4 72.6 48.7 35.3 168 金谷1号Jingu 1 91.5 86.8 34.2 19.5 178 晋谷21 Jingu 21 82.6 79.6 42.5 38.9