黄淮南片麦区新育成品种(系)中3个矮秆基因分子标记检测及其与农艺性状的关系
曹廷杰,张玉娥,胡卫国,杨剑,赵虹,王西成,周艳杰,赵群友,李会群

Detection of Three Dwarfing Genes in the New Wheat Cultivars (Lines) Developed in South Huang-Huai Valley and Its Association with Agronomic Traits
Tingjie Cao,Yu’e Zhang,Weiguo Hu,Jian Yang,Hong Zhao,Xicheng Wang,Yanjie Zhou,Qunyou Zhao,Huiqun Li
表1 用于检测RhtB1-b、RhtD1-b及Rht8的分子标记
Table 1 Molecular markers for detection of RhtB1-b、RhtD1-b and Rht8
检测基因名称
ID of detection gene
正向引物序列(5′-3′)
Sequence of forward primer (5′-3′)
反向引物序列(5′-3′)
Sequence of reverse primer (5′-3′)
退火温度(℃)
Annealing temperature
RhtB1-b TCTCCTCCCTCCCCACCCAAC CATCCCCATGGCCATCTCGAGCTA 63
RhtD1-b CGCGCAATTATTGGCCAGAGATAG CCCCATGGCCATCTCGAGCTGCTA 63
Rht8 CTCCCTGTACGCCTAAGGC CTCGCGCTACTAGCCATTG 55