黄淮南片麦区新育成品种（系）中3个矮秆基因分子标记检测及其与农艺性状的关系

曹廷杰,张玉娥,胡卫国,杨剑,赵虹,王西成,周艳杰,赵群友,李会群

Detection of Three Dwarfing Genes in the New Wheat Cultivars (Lines) Developed in South Huang-Huai Valley and Its Association with Agronomic Traits

Tingjie Cao,Yu’e Zhang,Weiguo Hu,Jian Yang,Hong Zhao,Xicheng Wang,Yanjie Zhou,Qunyou Zhao,Huiqun Li

表1 用于检测RhtB1-b、RhtD1-b及Rht8的分子标记

Table 1 Molecular markers for detection of RhtB1-b、RhtD1-b and Rht8

检测基因名称 ID of detection gene 正向引物序列(5′-3′) Sequence of forward primer (5′-3′) 反向引物序列(5′-3′) Sequence of reverse primer (5′-3′) 退火温度(℃) Annealing temperature RhtB1-b TCTCCTCCCTCCCCACCCAAC CATCCCCATGGCCATCTCGAGCTA 63 RhtD1-b CGCGCAATTATTGGCCAGAGATAG CCCCATGGCCATCTCGAGCTGCTA 63 Rht8 CTCCCTGTACGCCTAAGGC CTCGCGCTACTAGCCATTG 55