基于qPCR和LAMP技术的马铃薯晚疫病菌快速检测方法
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祝菊澧,梁静思,张佩,王伟伟,林桐司骐,谢欣娱,苏瑞,唐唯
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A Rapid Detection Method for Potato Late Blight Caused by Phytophthora infestans Based on qPCR and LAMP Assays
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Juli Zhu,Jingsi Liang,Pei Zhang,Weiwei Wang,Tongsiqi Lin,Xinyu Xie,Rui Su,Wei Tang
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表1 用于qPCR和LAMP检测卵菌及其他马铃薯相关病原菌菌株信息
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Table 1 Strain information of oomycetes and other potato related pathogens for qPCR and LAMP detection
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分类
Clossify | 编号
No. | 菌株
Strain | 种
Species | 寄主(分离部位)
Hosts (Isolation source) | 来源
Source | ITS序列
ITS sequence | | 卵菌 | 1 | 110P | Phytophthora infestans | Solanum tuberosum (leaf) | 中国云南 | LS479123 | | Oomycetes | 2 | XD1213 | P. infestans | S. tuberosum(leaf) | 中国云南 | LS479124 | | 3 | XD15 | P. infestans | S. tuberosum(leaf) | 中国云南 | LS479193 | | 4 | JC1207 | P. infestans | S. tuberosum(leaf) | 中国云南 | LS479126 | | 5 | XD1314 | P. infestans | S. tuberosum(leaf) | 中国云南 | LS479127 | | 6 | XA-4 | P. infestans | S. tuberosum(tuber) | 中国云南 | LS479171 | | 7 | DN111 | P. infestans | S. tuberosum(unknown) | 日本北海道 | LS479169 | | 8 | 80029 | P. infestans | S. tuberosum(unknown) | 荷兰瓦格宁根 | LS479173 | | 9 | 88069
| P. infestans
| Lycopersicon esculentum(leaf) | 荷兰瓦格宁根 | LS479172
| | 10 | Pds13 | P. sojae | Glycine max(leaf) | 中国云南 | LS479895 | | 11 | Ddr1231 | P. parasitica | Nicotiana tabacum (leaf) | 中国云南 | LS479896 | | 12 | LL2480 | P. capsica | Capsicum annuum(fruit) | 中国云南 | LS479897 | | 13 | Fuz37 | Pythium acanthicum | Saccharum officiarum L. (root) | 中国云南 | LS479899 | | 真菌 | 14 | AT1289 | Alternaria alternate | S. tuberosum(leaf) | 中国云南 | LS479189 | | Fungus | 15 | Hz1177 | Synchytrium endobioticum | S. tuberosum(root) | 中国云南 | LS479191 | | 16 | Hz1608 | Fusarium solani | S. tuberosum(leaf) | 中国云南 | LS479188 | | 细菌 | 17 | Hh1503
| Clavibacter michiganensissubsp. Sepedonicus | S. tuberosum(tuber) | 中国云南 | LS479190
| | Bacteria | 18 | Hhrf1
| Pectobacterium carotovorum subsp.Carotovorum | S. tuberosum(tuber)
| 中国云南 | LS479898
| | 19 | Hhqk1 | Ralstonia solanacearum | S. tuberosum(stem) | 中国云南 | LS479192 |
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