基于qPCR和LAMP技术的马铃薯晚疫病菌快速检测方法

祝菊澧,梁静思,张佩,王伟伟,林桐司骐,谢欣娱,苏瑞,唐唯

A Rapid Detection Method for Potato Late Blight Caused by Phytophthora infestans Based on qPCR and LAMP Assays

Juli Zhu,Jingsi Liang,Pei Zhang,Weiwei Wang,Tongsiqi Lin,Xinyu Xie,Rui Su,Wei Tang

图3 qPCR特异性和灵敏度检测 A：qPCR特异性检测,1：P. infestans gDNA;2：加入P. infestans gDNA的混合溶液;3：不加P. infestans gDNA的混合溶液;4：ddH2O。B：灵敏度检测,利用P. infestans（1×10-6~1×10-1ng/μL）DNA模板,建立标准曲线;E：扩增效率（103%）。C：在有寄主存在条件下的灵敏度检测,在有寄主存在条件下,利用引物infnew1,以P. infestans DNA浓度梯度（1×10-6~1×10-1ng/μL）建立标准曲线,E：扩增效率（108%）

Fig.3 Specific test and sensitive test by qPCR for species A: P. infestans species specific test by qPCR, 1: P. infestans gDNA; 2: Mixed solution with P. infestans gDNA; 3: Mixed solution but without P. infestans gDNA; 4: ddH2O. B: Sensitive test, sensitive test profiles of 10-fold serial dilutions of P. infestans (1×10-6~1×10-1ng/μL) to create standard curve; E: Reaction efficiency (103%). C: Sensitive test with the presentation of host DNA, quantitative real-time PCR (qPCR) amplification profiles of 10-fold serial dilutions of P. infestans (1×10-6~1×10-1ng/μL) using infnew1 primers with host gDNA; E: Reaction efficiency (108%)