基于qPCR和LAMP技术的马铃薯晚疫病菌快速检测方法
祝菊澧,梁静思,张佩,王伟伟,林桐司骐,谢欣娱,苏瑞,唐唯

A Rapid Detection Method for Potato Late Blight Caused by Phytophthora infestans Based on qPCR and LAMP Assays
Juli Zhu,Jingsi Liang,Pei Zhang,Weiwei Wang,Tongsiqi Lin,Xinyu Xie,Rui Su,Wei Tang
图4 LAMP特异性及灵敏度检测
A:19株菌株的LAMP反应;B:SYBR Green染色后在紫外线下观察阳性扩增的亮度变化;C:2.0%琼脂糖凝胶电泳检测LAMP产物
Fig.4 Specific test and sensitive test by LAMP for species
A: LAMP using 19 isolates; B: Observation of intensity change of positive amplification by SYBR Green staining under ultraviolet light; C: LAMP products analyzed by 2.0% agarose gel