水稻苗期耐低温基因COLD1新功能标记的设计与验证

田孟祥,宫彦龙,张时龙,何友勋,雷月,余本勋,余莉,李佳丽,张大双,叶永印

Design and Verification of New Functional Marker of Chilling-Tolerance COLD1 Gene in Rice Seedling Stage

Mengxiang Tian,Yanlong Gong,Shilong Zhang,Youxun He,Yue Lei,Benxun Yu,Li Yu,Jiali Li,Dashuang Zhang,Yongyin Ye

表1 COLD1基因功能标记的引物序列

Table 1 The primer sequences of functional marker for COLD1 gene

引物Primer 序列（5′-3′）Sequence (5′-3′) COLD1-O-F CATTTCCCCATGCCTTCTCC COLD1-O-R CAACTGTCCCAACGATACGC COLD1-I-F1 CCTGGCTTACAGGGAAATTGATGAGAT COLD1-I-F2 CTGGCTTACAGGGAAATTGATGAGAC COLD1-I-R GAGCTGCCTTTCCAATGTTTTGATGTTCT