水稻苗期耐低温基因COLD1新功能标记的设计与验证

田孟祥,宫彦龙,张时龙,何友勋,雷月,余本勋,余莉,李佳丽,张大双,叶永印

Design and Verification of New Functional Marker of Chilling-Tolerance COLD1 Gene in Rice Seedling Stage

Mengxiang Tian,Yanlong Gong,Shilong Zhang,Youxun He,Yue Lei,Benxun Yu,Li Yu,Jiali Li,Dashuang Zhang,Yongyin Ye

图1 COLD1基因功能标记设计策略 序列中字母下划线表示单核苷酸差异位点SNP2;省略号表示相同的碱基;箭头表示引物位置和扩增方向;方框内为引入的错配碱基

Fig.1 Design strategies for the primers to detect COLD1 gene The underlines indicate the functional polymorphism SNP2 in the COLD1 gene; the ellipses indicate the same base; the arrows indicate the position and amplification direction of the corresponding primers; the letters in the box present the mismatched bases