黑龙江省稻瘟病菌无毒基因的检测

孟峰, 张亚玲, 靳学慧

Detection of Avirulence Genes of Magnaporthe oryzea in Heilongjiang Province

Feng Meng, Yaling Zhang, Xuehui Jin

表1 用于扩增稻瘟病菌无毒基因的引物

Table 1 The primers for amplification of Magnaporthe oryzea avirulence genes

无毒基因 Avirulence gene 引物序列 (5′-3′) Primer sequence (5′-3′) 目的片段长度 Length of targeted fragment (bp) ACE1 F: GTGGAATCTTGTGGTGCTACGG R: ACGGGAACATCGACGGAAAG 1 057 AVR-Pi9 F: CGGCAGGTGGGTTTATCATC R: CCAAAGCAGACAGCGACTCTA 1 070 AVR-Pia F: TAGCCATTTCGGTCTCAT R: CGGGTAAACCCTGTCATA 1 597 AVR-Pii F: TATGCAGGCCCAAATCCGTAG R: CCCGCAATAGTCCATAACTGTCC 526 AVR1-CO39 F: CCTTGCCGCTACTGAAAC R: CCCTATCGCAGAGGAACA 419