云南药用野生稻高质量染色体DNA的制备

李娥贤, 殷富有, 张敦宇, 陈越, 余腾琼, 雷涌涛, 肖素勤, 程在全, 柯学

Extraction of High-Quality Chromosome DNA in Yunnan Wild Rice Oryza officinalis

Li Exian, Yin Fuyou, Zhang Dunyu, Chen Yue, Yu Tengqiong, Lei Yongtao, Xiao Suqin, Cheng Zaiquan, Ke Xue

图2 药用野生稻叶片细胞核的低熔点琼脂糖包埋 A.核重悬;B.琼脂糖在冰上凝固;C.包埋块凝固于医用注射器中;D.包埋块切成圆片;E.嵌入式块清洗;F.包埋块洗涤

Fig.2 Nucleus embedding of O. officinalis by low-melting point agarose A. Nuclear resuspension; B. Agarose solidified on the ice; C. Embedding block is solidified in the medical syringe; D. Cut into a circular piece; E. Embedded block washing; F. Embedded block after washing