云南药用野生稻高质量染色体DNA的制备

李娥贤, 殷富有, 张敦宇, 陈越, 余腾琼, 雷涌涛, 肖素勤, 程在全, 柯学

Extraction of High-Quality Chromosome DNA in Yunnan Wild Rice Oryza officinalis

Li Exian, Yin Fuyou, Zhang Dunyu, Chen Yue, Yu Tengqiong, Lei Yongtao, Xiao Suqin, Cheng Zaiquan, Ke Xue

图6 BIBAC质粒及药用野生稻染色体大片段回收 A. BIBAC质粒及酶解,B.染色体大片段回收;M. λDNA/HindⅢ marker (A)和D2000 marker (B)

Fig.6 BIBAC vector and large-fragment DNA recovery of O.officinalis A. BIBAC and digested by BamHⅠ, B. Target DNA recovery for BamHⅠ;M. Yeast chromosome PFG marker (A) and D2000 marker (B)