苦荞鼠李糖基转移酶FtF3GT1基因的克隆与转化毛状根研究

卢晓玲, 何铭, 张凯旋, 廖志勇, 周美亮

Study on the Cloning and Transformation of Rhamnose Transferase FtF3GT1 Gene in Tartary Buckwheat

Lu Xiaoling, He Ming, Zhang Kaixuan, Liao Zhiyong, Zhou Meiliang

表1 引物序列

Table 1 Primer sequence

引物名称Primer name 引物序列(5'-3') Primer sequence (5'-3') FtF3GT1-F(CDS) 5'-ATGGGAACCCAATCAAGC-3' FtF3GT1-R(CDS) 5'-CTACTGCTTACCAACCAAAC-3' pCAMBIA1307-FtF3GT1-F 5'-GGGGGCGGCCGCTCTAGAATGGGAACCCAATCAAGC-3' pCAMBIA1307-FtF3GT1-R 5'-AAGCTTGATATCGAATTCCTACTGCTTACCAACCAAAC-3' pCAMBIA3301-FtF3GT1-F 5'-GAATCCTTAAATGGTTCATATTACTATGCAATTAG-3' pCAMBIA3301-FtF3GT1-R 5'-TCTAGATTTGTAAAATGCTTGATTTGTTTCTTG-3' FtH3-QF 5'-GAAATTCGCAAGTACCAGAAGAG-3' FtH3-QR 5'-CCAACAAGGTATGCCTCAGC-3' FtF3GT1-QF 5'-TCAAATAAGCTCGCCTC-3' FtF3GT1-QR 5'-GCTGCATTTTGTCAAGAGCG-3' FtActin-QF 5'-GAAATTCGCAAGTACCAGAAGAG-3' FtActin-QR 5'-CCAACAAGGTATGCCTCAGC-3'