苦荞鼠李糖基转移酶FtF3GT1基因的克隆与转化毛状根研究

卢晓玲, 何铭, 张凯旋, 廖志勇, 周美亮

Study on the Cloning and Transformation of Rhamnose Transferase FtF3GT1 Gene in Tartary Buckwheat

Lu Xiaoling, He Ming, Zhang Kaixuan, Liao Zhiyong, Zhou Meiliang

图6 转基因根系的鉴定及GUS染色 A：FtF3GT1pro::GUS转基因毛状根鉴定图;M：DL 2 000 DNA标记;1：pCAMBIA3301:FtF3GT1pro::GUS质粒;2~4：转A4根系DNA;5~7：转pCAMBIA3301-35S::GUS根系DNA;8~11：转FtF3GT1pro::GUS根系DNA;B：MeJA处理FtF3GT1pro::GUS毛状根的GUS染色情况

Fig.6 Identification of transgenic roots and GUS staining A: Molecular identification of transgenic FtF3GT1pro::GUS hairy root lines. M: DL 2 000 DNA marker; 1: pCAMBIA3301:FtF3GT1pro::GUS plasmid; 2-4: transgenic lines DNA of A4 rhizogenes; 5-7: transgenic lines DNA of pCAMBIA3301-35S::GUS; 8-11: transgenic lines DNA of FtF3GT1pro::GUS; B: GUS staining of FtF3GT1pro::GUS hairy root treated with MeJA