苦荞鼠李糖基转移酶FtF3GT1基因的克隆与转化毛状根研究

卢晓玲, 何铭, 张凯旋, 廖志勇, 周美亮

Study on the Cloning and Transformation of Rhamnose Transferase FtF3GT1 Gene in Tartary Buckwheat

Lu Xiaoling, He Ming, Zhang Kaixuan, Liao Zhiyong, Zhou Meiliang

图7 苦荞毛状根的诱导过程和转基因根系的鉴定 A：苦荞毛状根的诱导过程;B：转基因根系的鉴定;M：DL 2 000 DNA标记;1：pCAMBIA1307-FtRT1质粒;2：天然的苦荞根系（WT）;3：转pCAMBIA1307空载体根系;4~8：转pCAMBIA1307-FtRT1根系DNA检测

Fig.7 Induction process of hairy roots of tartary buckwheat and identification of transgenic root lines A: Induction process of hairy roots of tartary buckwheat; B: Identification of transgenic root lines; M: DL 2 000 DNA Marker; 1: pCAMBIA1307-FtRT1 plasmid; 2: natural root of tartary buckwheat (WT); 3: pCAMBIA1307 transgenic root lines; 4-8: DNA detection of pCAMBIA1307-FtRT1 transgenic root lines