甜菜BvWRKY23基因的RNAi载体构建

李国龙, 吴海霞, 孙亚卿

Construction of RNAi Expression Vector of BvWRKY23 Gene in Sugar Beet

Li Guolong, Wu Haixia, Sun Yaqing

表1 RNAi片段及内含子扩增引物

Table 1 Amplifying primers of RNAi fragments and intron

引物类型Primer type 引物名称Primer name 序列(5'-3') Sequence (5'-3') 酶切位点Enzyme site P1 BvWRKY23-F1 CGGGATCCGAACCATTGAAGATTGAA BamHI BvWRKY23-R1 GCTCTAGAAGAGGTGGAGGGGTAGGA XbaI P2 BvWRKY23-F2 CGAGCTCGAACCATTGAAGATTGAA SacI BvWRKY23-R2 ATTTGCGGCCGCAGAGGTGGAGGGGTAGGA NotI 内含子Intron (RTM) RTM-F ACGTTGTAAGTCTATTTTTG RTM-R TCTATCTGCTGGGTCCAAATC