过表达FtbZIP5提高苦荞毛状根黄酮积累及其耐盐性

翁文凤, 伍小方, 张凯旋, 唐宇, 江燕, 阮景军, 周美亮

The Overexpression of FtbZIP5 Improves Accumulation of Flavonoid in the Hairy Roots of Tartary Buckwheat and Its Salt Tolerance

Weng Wenfeng, Wu Xiaofang, Zhang Kaixuan, Tang Yu, Jiang Yan, Ruan Jingjun, Zhou Meiliang

表1 引物序列汇总

Table 1 Summary of primer sequence

引物名称Primer 引物序列Primer sequence (5′-3′) 用途Function 1307-FtbZIP5-F CTAGAACTAGTGGATCCATGGGAAGTAACCTG 构建过表达载体 1307-FtbZIP5-R GCGGAGTACCCGGGTACCTCACCACACACCAG pAS-FtbZIP5-F TACCCAGCTTTGACTCATATGATGGGAAGTAACCTG 转录活性分析 pAS-FtbZIP5-R TTAGCTTGGCTGCAGGTCGACTCACCACACACCAG FtbZIP5-F ATGGGAAGTAACCTG 基因克隆 FtbZIP5-R TCACCACACACCAG TLF CTCAAGCAA TCAAGCA TTCTAC 转基因毛状根阳性检测 RT1-QF TCAAA TAAGCTCGCCTCCCA RT1-QR GCTGCA TTTTGTCAAGAGCG FtbZIP5-QF CAAGTTCCTTTGCAGTCGCC FtbZIP5-QR TAATCCCTGTGCAACCCACC FtCHS-QF AGACGCATGTGCGACAAATC FtCHS-QR CCACTCCTTGATGGCCTTCT FtCHI-QF GGCACAGTACTCTGAAAAGGT FtCHI-QR CTTCCCGAATGCAATCCTTAATG FtF3H-QF AACAGCAGCCGTTTGTCAAT FtF3H-QR TGCTCCTTAGCCAGCTTCTT FtPAL-QF ACAAGGCGTTACATGGAGGA FtPAL-QR CCAAGCTAGGGTTTCTCCCA