黑龙江省粳稻品种稻瘟病主效抗性基因鉴定与抗性评价

高清, 张亚玲, 周弋力, 于连鹏, 聂强, 靳学慧

Identification of Major Resistance Genes and Resistance Evaluation to Rice Blast in Japonica Rice Varieties in Heilongjiang Province

Gao Qing, Zhang Yaling, Zhou Yili, Yu Lianpeng, Nie Qiang, Jin Xuehui

表1 特异性分子标记的引物信息

Table 1 Primer information for specific molecular markers

抗性基因 Resistance gene 引物 Primer 引物序列 Primer sequence (5'-3') 片段大小 Fragment size (bp) 酶 Enzyme 参考文献 Reference Pita YL155-F AGCAGGTTATAAGCTAGGCC 1042(R) [7-9] YL87-R CTACCAACAAGTTCATCAAA YL183-F AGCAGGTTATAAGCTAGCTAT 1042(S) YL87-R CTACCAACAAGTTCATCAAA Pia Pia-F GTCACGACGACCATCCAGTCAGTAG 2196(R) [10] Pia-R AGGTAACAGGGAGTGTAGGAAATCA Piz-t Piz-t-F1 CTTCTATGCAGTCGTGAGATTGGTT 206(R) [10] Piz-t-R1 ATGCTACGATGGAAATCAACACAAT Piz-t-F2 TAAAAGATTTCTTGATTTCACTCGG 3154(R) Piz-t-R2 ACCTGATGGCTAATACTGAACAAGA Piz-t-F3 GGGCGATGATTTCAACTCACTTTTC 136(R) Piz-t-R3 GATCATCATGTCCCTCCGAGTCAGA Pib Pibdom-F GAACAATGCCCAAACTTGAGA 365(R) [11-13] Pibdom-R GGGTCCACATGTCAGTGAGC Lys145-F TCGGTGCCTCGGTAGTCAGT 803(S) Lys145-R GGGAAGCGGATCCTAGGTCT Pikm Ckm1-F TGAGCTCAAGGCAAGAGTTGAGGA 174 (R)/213 (S) [14-15] Ckm1-R TGTTCCAGCAACTCGATGAG Ckm2-F CAGTAGCTGTGTCTCAGAACTATG 290 (R)/332 (S) Ckm2-R AAGGTACCTCTTTTCGGCCAG Pi9 Pi9-F GCTGTGCTCCAAATGAGGAT 291 (R)/397(S) [16] Pi9-R GCGATCTCACATCCTTTGCT Pii Pii-4SNP-F TCCAATGCTTCTGAAAGGTAGC 353/240+113 PvuⅡ [17] Pii-4SNP-R TGGAAACATGAACCCATATCC Pid3 Pid3-F TACTACTCATGGAAGCTAGTTCTC 658 BamHⅠ [18] Pid3-R ACGTCACAAATCATTCGCTC