薰衣草芳樟醇合酶基因的克隆、表达及酶活性检测

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Cloning, Expression and Enzyme Activity Detection of Linalool Synthase Gene in Lavender

Gong Lintao, Su Xiujuan, Liao Yan, Keremuhan·Wusiman , Zhou Di, Yin Songsong

图10 重组质粒pET-28a(+)-LIS1和pET-28a(+)-LIS2的双酶切鉴定 M：Marker 15 000;1：pET-28a(+)-LIS1质粒经EcoRⅠ、Hind Ⅲ双酶切;2：pET-28a(+)-LIS1线性质粒;3：pET-28a(+)-LIS2质粒经NdeⅠ、EcoRⅠ双酶切;4：pET-28a(+)-LIS2线性质粒

Fig.10 Identification of recombinant plasmid pET-28a(+)-LIS1 and pET-28a(+)-LIS2 by double enzyme digestion M: Marker 15000; 1: pET-28a(+)- LIS1 digested by double enzyme EcoRⅠand Hind Ⅲ; 2: pET-28a(+)-LIS1 linear plasmid; 3: pET-28a(+)- LIS2 digested by double enzyme NdeⅠ and EcoRⅠ; 4: pET-28a(+)-LIS2 linear plasmid