薰衣草芳樟醇合酶基因的克隆、表达及酶活性检测

龚林涛, 苏秀娟, 廖燕, 克热木汗·吾斯曼, 周迪, 尹松松

Cloning, Expression and Enzyme Activity Detection of Linalool Synthase Gene in Lavender

Gong Lintao, Su Xiujuan, Liao Yan, Keremuhan·Wusiman , Zhou Di, Yin Songsong

图11 LIS1和LIS2重组蛋白原核表达分析 M：蛋白ProteinRuler®Ⅰ,下同;1：pET-28a(+)未诱导;2：pET-28a(+)诱导后;3：pET-28a(+)-LIS2蛋白诱导前;4：pET-28a(+)-LIS2蛋白未诱导;5：pET-28a(+)-LIS2蛋白诱导后;6：pET-28a(+)未诱导;7：pET-28a(+)诱导后;8：pET-28a(+)-LIS1蛋白诱导前;9：pET-28a(+)-LIS1蛋白未诱导;10：pET-28a(+)-LIS1蛋白诱导后

Fig.11 Prokaryotic expression analysis of LIS1 and LIS2 recombinant proteins M: ProteinRuler®Ⅰ, the same below; 1: transetta with pET-28a(+) uninduced; 2: transetta with pET-28a(+) induced by IPTG; 3: transetta with pET-28a(+)-LIS2 before induction; 4: transetta with pET-28a(+)-LIS2 uninduced; 5: transetta with pET-28a(+)-LIS2 by IPTG; 6: transetta with pET-28a(+) uninduced; 7: transetta with pET-28a(+) induced by IPTG; 8: transetta with pET-28a(+)-LIS1 before induction; 9: transetta with pET-28a(+)-LIS1 uninduced; 10: transetta with pET-28a(+)-LIS1 by IPTG