优异糯高粱不育系吉5535A的创制与应用

陈冰嬬, 于淼, 石贵山, 王江红, 唐玉劼, 徐晨, 李海青, 徐宁, 周紫阳, 王鼐

Breeding and Application of Elite Waxy Sorghum Male Sterile Line “Ji 5535A”

Chen Bingru, Yu Miao, Shi Guishan, Wang Jianghong, Tang Yujie, Xu Chen, Li Haiqing, Xu Ning, Zhou Ziyang, Wang Nai

图3 吉5535A、吉2055A和吉521A的Waxy基因测序结果
(a) 利用Wx^a-F、Wx-F和Wx-R 3条引物扩增，琼脂糖电泳结果显示吉5535A扩增片段为523bp，不是Wx^a类型突变，是Wx-WT、Wx^b、Wx^c和Wx^d 中的一种。(b) 利用引物Wx-F2和Wx-R2 PCR扩增后测序，结果显示吉5535A在CDS区804位G突变成T为Wx^b类型突变

Fig.3 The results of Waxy sequencing of Ji 5535A, Ji 2055A and Ji 521A
(a) the agarose electrophoresis result showed that the amplified fragment of Ji 5535A was 523bp by Wx^a-F、Wx-F and Wx-R primers, the Waxy allele mutation of Ji 5535A was not Wx^a and was one of Wx-WT、Wx^b、Wx^c and Wx^d. (b) Sequenced after PCR amplification using the primers Wx-F2 and Wx-R2, the sequencing result showed that the Waxy allele mutation of Ji 5535A was Wx^b type and 804 site G mutated to T in CDS