苦荞抗立枯病基因FtTIR的克隆及功能鉴定

陈媛媛, 李光胜, 刘洋, 何毓琦, 周美亮, 方正武

Molecular Cloning and Functional Identification of Resistance Gene FtTIR of Tartary Buckwheat to Blight

Chen Yuanyuan, Li Guangsheng, Liu Yang, He Yuqi, Zhou Meiliang, Fang Zhengwu

表1 引物序列汇总

Table 1 Summary of primer sequence

引物Primer 引物序列（5′-3′）Primer sequence (5′-3′) 用途Function FtTIR-F ATGCAACGTCCAGTAGCCAAA 基因克隆 FtTIR-R TTATTTGCTGTTTTCTTCAACCTCG FtTIR-qPCR-F GCCTTCTTGCTAATGGG qRT-PCR FtTIR-qPCR-R GCTTACAACAACACCTCC AtPR1-qPCR-F TCATGGCTAAGTTTGCTTCC AtPR1-qPCR-R AATACACACGATTTAGCACC FtH3-qPCR-F GAAATTCGCAAGTACCAGAAGAG qRT-PCR内参基因 FtH3-qPCR-R CCAACAAGGTATGCCTCAGC Atactin7-qPCR-F TCCATGAAACAACTTACAACTCCATCA Atactin7-qPCR-R CATCGTACTCACTCTTTGAAATCCACA 121-FtTIR-F GAGAACACGGGGGACTCTAGAATGCAACGTCCAGTAGCCAAA 构建过表达载体 121-FtTIR-R CATACCTCCTCCTCCGGATCCTTTGCTGTTTTCTTCAACCTCG 121F TGACGTAAGGGATGACGCAC pBI121通用引物