温光敏两系杂交小麦杂交种纯度的表型和标记检测比较

李宏生, 李绍祥, 杨忠慧, 杨家李, 刘琨, 熊世安, 李富乾, 郭辉, 杨木军

Comparison ofPhenotype and Marker Detection in Seed Purity of Thermo-Photo Sensitive Two-Line WheatHybrids

Li Hongsheng, Li Shaoxiang, Yang Zhonghui, Yang Jiali, Liu Kun, Xiong Shian, Li Fuqian, Guo Hui, Yang Mujun

表1 SSR标记引物序列

Table 1 Primersequences of SSR markers

引物名称Primer name 染色体分布Chromosome location 引物序列（5′-3′）The sequence of primer(5′-3′) barc324 3A F：CCAATTCTGCCCATAGGTGA R：GAGGAAATAAGATTCAGCCAACTG barc164 3B F：TGCAAACTAATCACCAGCGTAA R：CGCTTTCTAAAACTGTTCGGGATTTCTAA cfa2028 7A F：TGGGTATGAAAGGCTGAAGG R：ATCGCGACTATTCAACGCTT cfa2123 7A F：CGGTCTTTGTTTGCTCTAAACC R：ACCGGCCATCTATGATGAAG gwm155 3A F：CAATCATTTCCCCCTCCC R：AATCATTGGAAATCCATATGCC gwm161 3D F：GATCGAGTGATGGCAGATGG R：TGTGAATTACTTGGACGTGG gwm304 5A F：AGGAAACAGAAATATCGCGG R：AGGACTGTGGGGAATGAATG gwm610 4A F：AGGAAACAGAAATATCGCGG R：AGGACTGTGGGGAATGAATG