基于叶绿体SSR分子标记的苦参种质资源遗传多样性分析

宋芸, 张鑫瑞, 贺嘉欣, 李政, 孙哲, 李澳旋, 乔永刚

Genetic Diversity Analysis of Sophora flavescens Ait. Germplasm Resources Based on cpSSR Markers

Song Yun, Zhang Xinrui, He Jiaxin, Li Zheng, Sun Zhe, Li Aoxuan, Qiao Yonggang

表2 cpSSR引物信息

Table 2 Information of cpSSR primer

引物名称 Primer name 正向序列 Forward sequence (5′-3′) 反向序列 Reverse sequence (5′-3′) 产物长度 Product length (bp) 退火温度 Annealing temperature (℃) KS1 AAACCGACACGGATTACTCG CCCAATGTACCCTGATACGG 271 54.8 KS2 CCCCCAATCCTTCTTTGATT ATTGGCTGTTCGTCAATTCC 227 52.1 KS3 ATGAGGTTGTGAATCCGAGG CGATTCGATAAACGGCTCAT 246 54.5 KS4 ACAGGATTTGAACCCGTGAC CCTTCCTTATAATTTCATATCCTTCC 223 55.2 KS5 ACCTTCCCGGAGACTGAACT AAAGCTTTTGTTTCGGCTCA 276 58.2 KS6 TCCAATAACCATCCTTCCCTT GAGTTTTCACACCGGAAAGC 213 52.9 KS7 ACTCCTTTGATGGGTGTTGC ACAAAGAAATTCCACGGTCG 228 55.5 KS8 GGAGCACGGAATATCGAAAA CCAGAACCACGATGATTGAA 194 52.3 KS9 AACAGGCTCCGTAAGATCCA CATCTCAGACCTTGCGATTG 268 55.7 KS10 GCCTACGGATCAATCGACAT TGACCACGAAAGTCAAAATGA 229 54.5 KS11 TTTTCACCTCATACGGCTCC TGGTTATATATGGATTGCTAAAATTG 225 54.7 KS12 TTGATGCCTTGATCGAATGA TGAGCCAGGATCGAACTCTC 229 51.1 KS13 GAATCGACAGATCCACCGAC GAATCGACAGATCCACCGAC 250 55.6 KS14 TGAACTAATTGATTTGATTATTTTCCA TTTGGAACTGCCATCCAACT 206 47.9 KS15 TCAAGGGGTTCTCACAAACA ATTCTCGGTTGACAGGGTTG 145 53.6 KS16 AGAAGATTAAGGAACCCCCG GCATGAAACAACTCGAAGCA 245 54.0 KS17 CCGTGGGACTTAAGGAATGA CTCAAAGCAAAGCCAAGGAG 246 54.2 KS18 GGGGGAAACGGATACTCAAT TTCTTCTGCAGTACCTCGCC 270 54.3