基于叶绿体SSR分子标记的苦参种质资源遗传多样性分析
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宋芸, 张鑫瑞, 贺嘉欣, 李政, 孙哲, 李澳旋, 乔永刚
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Genetic Diversity Analysis of Sophora flavescens Ait. Germplasm Resources Based on cpSSR Markers
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Song Yun, Zhang Xinrui, He Jiaxin, Li Zheng, Sun Zhe, Li Aoxuan, Qiao Yonggang
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表2 cpSSR引物信息
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Table 2 Information of cpSSR primer
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引物名称 Primer name | 正向序列 Forward sequence (5′-3′) | 反向序列 Reverse sequence (5′-3′) | 产物长度 Product length (bp) | 退火温度 Annealing temperature (℃) | KS1 | AAACCGACACGGATTACTCG | CCCAATGTACCCTGATACGG | 271 | 54.8 | KS2 | CCCCCAATCCTTCTTTGATT | ATTGGCTGTTCGTCAATTCC | 227 | 52.1 | KS3 | ATGAGGTTGTGAATCCGAGG | CGATTCGATAAACGGCTCAT | 246 | 54.5 | KS4 | ACAGGATTTGAACCCGTGAC | CCTTCCTTATAATTTCATATCCTTCC | 223 | 55.2 | KS5 | ACCTTCCCGGAGACTGAACT | AAAGCTTTTGTTTCGGCTCA | 276 | 58.2 | KS6 | TCCAATAACCATCCTTCCCTT | GAGTTTTCACACCGGAAAGC | 213 | 52.9 | KS7 | ACTCCTTTGATGGGTGTTGC | ACAAAGAAATTCCACGGTCG | 228 | 55.5 | KS8 | GGAGCACGGAATATCGAAAA | CCAGAACCACGATGATTGAA | 194 | 52.3 | KS9 | AACAGGCTCCGTAAGATCCA | CATCTCAGACCTTGCGATTG | 268 | 55.7 | KS10 | GCCTACGGATCAATCGACAT | TGACCACGAAAGTCAAAATGA | 229 | 54.5 | KS11 | TTTTCACCTCATACGGCTCC | TGGTTATATATGGATTGCTAAAATTG | 225 | 54.7 | KS12 | TTGATGCCTTGATCGAATGA | TGAGCCAGGATCGAACTCTC | 229 | 51.1 | KS13 | GAATCGACAGATCCACCGAC | GAATCGACAGATCCACCGAC | 250 | 55.6 | KS14 | TGAACTAATTGATTTGATTATTTTCCA | TTTGGAACTGCCATCCAACT | 206 | 47.9 | KS15 | TCAAGGGGTTCTCACAAACA | ATTCTCGGTTGACAGGGTTG | 145 | 53.6 | KS16 | AGAAGATTAAGGAACCCCCG | GCATGAAACAACTCGAAGCA | 245 | 54.0 | KS17 | CCGTGGGACTTAAGGAATGA | CTCAAAGCAAAGCCAAGGAG | 246 | 54.2 | KS18 | GGGGGAAACGGATACTCAAT | TTCTTCTGCAGTACCTCGCC | 270 | 54.3 |
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