水稻胚乳突变体cse-2的表型分析及基因定位

孙家猛, 高原, 陈虎, 花芹, 林泉祥, 陈庆全, 李金才, 张海涛

Phenotypic Analysis and Gene Mapping of Rice Mutant Chalkiness and Shrunken Endosperm-2

Sun Jiameng, Gao Yuan, Chen Hu, Hua Qin, Lin Quanxiang, Chen Qingquan, Li Jincai, Zhang Haitao

图4 CSE-2基因的精细定位 a~d：横线上方为定位所用的分子标记，横线下方为交换单株数，浅蓝色箭头表示候选基因；e：候选基因CSE-2结构图，浅蓝色框代表外显子，其中间空白部分代表内含子，红色箭头分别表示起始密码子、终止密码子、突变位点，黑线框内容表示突变位点；f：目的基因反向测序峰图，红线框内为突变位点；g：部分蛋白质序列比对，无义突变引起的氨基酸缺失。

Fig.4 Fine mapping of CSE-2 gene a-d: the numbers above and beneath the bold lines represent the molecular markers and recombinants, respectively, and the light blue arrow represent the candidate gene; e: the structure diagram of candidate gene CSE-2, the light blue box represent the exon, the blank part represent the intron, the red arrows represent the start codon, stop codon and mutation site, respectively, and the content in the black box represent the mutation; f: target gene reverse sequencing peak map, with mutated sites in the red line box; g: partial protein sequence alignment near the none-sense mutation site.