基于SSR分子标记的晋北地区引进马铃薯种质资源遗传多样性分析

毛向红, 范向斌, 白小东, 卢瑶, 杜培兵

Genetic Diversity Analysis of Introduced Potato Germplasm Resources in Northern Shanxi Province Based on SSR Molecular Markers

Mao Xianghong, Fan Xiangbin, Bai Xiaodong, Lu Yao, Du Peibing

表2 用于马铃薯PCR扩增的SSR引物

Table 2 The SSR primers used for PCR amplification in potato

引物 Primer 正向引物 Forward primer (5′-3′) 反向引物 Reverse primer (5′-3′) 退火温度 Annealing temperature (℃) 片段大小 Fragment length (bp) STM1049 CTACCAGTTTGTTGATTGTGGTG AGGGACTTTAATTTGTTGGACG 57 184~254 STM2022 GCGTCAGCGATTTCAGTACTA TTCAGTCAACTCCTGTTGCG STM1053 TCTCCCCATCTTAATGTTTC CAACACAGCATSCAGATCATC 53 184~244 STM3023a AAGCTGTTACTTGATTGCTGCA GTTCTGGCATTTCCATCTAGAGA STPoAc58 TTGATGAAAGGAATGCAGCTTGTG ACGTTAAAGAAGTGAGAGTACGAC 53 168~184 STM0019a AATAGGTGTACTGACTCTCAATG TTGAAGTAAAAGTCCTAGTATGTG STM2013 TTCGGAATTACCCTCTGCC AAAAAAAGAACGCGCACG 50 169~201 STM1104 TGATTCTCTTGCCTACTGTAATCG CAAAGTGGTGTGAAGCTGTGA STM3012 CAACTCAAACCAGAAGGCAAA GAGAAATGGGCACAAAAAACA 57 203~277 STM1106 TCCAGCTGATTGGTTAGGTTG ATGCGAATCTACTCGTCATGG STM0037 AATTTAACTTAGAAGATTAGTCTC ATTTGGTTGGGTATGATA 47 155~241 STM0030 AGAGATCGATGTAAAACACGT GTGGCATTTTGATGGATT