甘蓝型油菜LPAT家族基因鉴定与表达模式分析

李坤杰, 刘念, 丁磊, 朱燕, 蒙大庆, 范其新, 李迎春, 陈军

Identification and Expression Pattern Analysis of LPAT Family Genes in Brassica napus

Li Kunjie, Liu Nian, Ding Lei, Zhu Yan, Meng Daqing, Fan Qixin, Li Yingchun, Chen Jun

表1 试验所用引物

Table 1 Primers used in this test

基因名称Gene name 上游引物（5′-3′）Forward primer (5′-3′) 下游引物（5′-3′）Reverse primer (5′-3′) BnLPAT1-A AGCGTTGGATGCATCTGGTTC GGTTATTGTCAGTTCCTTGA BnLPAT1-C GCACAATATTAGAGAGTGTT GGTTATTGTCAGTTCCTTGA BnLPAT4-A ATGGCAGCAGCAGTGATTGT GGTCGAACGAGGACATAGCA BnLPAT4-C ATGGCAGCAGCAGCAGTGAT CAGAGGTCGAATGAGGACAT BnLPAT12-A TGAGGCTGATCTTCCAGCATT AGCAGAACACTCTGCATCGCT BnLPAT14-A GTATCATATATCAGTGGGCA ACTGTGCCAGATACACCCTT BnLPAT14-C TGTATCATATGTCTGCTTCT ACTGTGCCAGATACACCCTT BnLPAT19-A AGTATTTGCGGATAATGAGAC AACCTGACCTCTGAGCGAGA BnLPAT19-C AAGTGTTTGCTGATAATGAG ACCTGATCTCTGAGCCAGAAT BnLPAT20-A TCACATCCTCCATATTATAG TCAAGTTCGAAGAACGAGCA BnLPAT20-C CATCCTCCTCCATATTATAGC AGAACGACCAAAGCAACATC BnLAPT22-C TGTATCATATGTCTGCTTCT CCTCTCGTTGGACATAGACA BnActin7 TGGGTTTGCTGGTGACGAT TGCCTAGGACGACCAACAATACT