高粱Dof基因家族鉴定及表达分析

付森杰, 宋迎辉, 朱灿灿, 叶珍言, 秦娜, 代书桃, 刘海霞, 王春义, 李君霞

Identification and Expression Analysis of Dof Gene Family in Sorghum

Fu Senjie, Song Yinghui, Zhu Cancan, Ye Zhenyan, Qin Na, Dai Shutao, Liu Haixia, Wang Chunyi, Li Junxia

表1 高粱Dof基因的qRT-PCR扩增所用引物序列

Table 1 Primer sequences for qRT-PCR amplification of Dof genes of sorghum

基因Gene 登录号Accession number 上游引物序列Upstream primer sequence 下游引物序列Downstream primer sequence SbDof-1 Sobic.001G034300 GTCGTCCAAGTCAAACTCGTC TCAGTCCGGGGAACCCTAA SbDof-4 Sobic.001G179300 CGACTACCACCACCAGCAG CAGCTTCCAGTGCAGATCCT SbDof-8 Sobic.001G489900 CTCATCAAGCTGTTCGGCAAG GTCTTCAGTGTCCGCAACCT SbDof-10 Sobic.002G313800 TCCATGAACCCTGCGGTTAG TAGAAGAGCGTGGCAATGGA SbDof-11 Sobic.002G421900 GAGCTGCGTCCCCTCG ACCCTCCGTCTTGGCTATCT SbDof-13 Sobic.003G121400 CCTGGGATCAAGCTCTTCGG GGCGCATCGACCTTCATTTC SbDof-24 Sobic.007G223200 ACAACTACAACACCTCCCAGC GTGTCTGTGACACCACTGGC SbDof-25 Sobic.008G001700 TCTCAGGCTCTGGTTCCTCT CGGGAGCACATTGGGGAATA Actin LOC110436378 TGAAGTGCGACGTGGATATTAGGA GCTGGAATGTGCTGAGAGATGC