突变NtJAZ1基因获得高烟碱烤烟新材料

高玉龙, 赵璐, 王丙武, 孔光辉, 王亚辉, 刘剑金, 段杰, 吴兴富, 李青, 者开明

Mutating NtJAZ1 Gene to Obtain High Nicotine Flue-Cured Tobacco New Material

Gao Yulong, Zhao Lu, Wang Bingwu, Kong Guanghui, Wang Yahui, Liu Jianjin, Duan Jie, Wu Xingfu, Li Qing, Zhe Kaiming

表1 筛选获得的NtJAZ1基因突变体信息

Table 1 Information of NtJAZ1 gene mutants screened

突变体编号 Number of mutants 核苷酸突变 Nucleotide mutation 氨基酸突变体 Amino acid mutation 突变位点所在结构域 The domain of the mutation site J2 C305T T102I ZIM J3 C346T P116S ZIM J4 G584A R195K Jas J5 C650T T217I C端 J6 G652A E218K C端 J8 G500A R167Q ZIM和Jas间隔区 J9 C708T A203V Jas J10 C616T Q206* Jas J11 C392T T131I ZIM和Jas间隔区 J14 C473T T158I ZIM和Jas间隔区 J15 G372A M124I ZIM J16 内含子 内含子