玉米ZmCaM1基因的克隆和表达分析

贺新春, 杜何为, 黄敏

Cloning and Expression Analysis of Maize ZmCaM1 Gene

He Xinchun, Du Hewei, Huang Min

表1 PCR和qRT-PCR引物的信息

Table 1 Informations of primers for PCR and qRT-PCR

引物名称Primer name 基因ID Gene ID 上游引物Forward primer (5′-3′) 下游引物Reverse primer (5′-3′) ZmCaM1 Zm00001d007194 ATGGCGGACCAGCTCACC TCACTTGGCCATCATGACCTT qZmCaM1 Zm00001d007194 TCAAATCGCTCCCTGCCTCT GACCCAGTGATCGCATGACA BK-CaM1 Zm00001d007194 AGGCCGAATTCCCGGGGATCCAT GGCGGACCAACTCACCG CCGCTGCAGGTCGACGGATCCTCAC TTGGCCATCATGACCTT AD-AP1G1 Zm00001d045195 GCCATGGAGGCCAGTGAATTCATGG ACCTCGCCATCAATCC ACGATTCATCTGCAGCTCGAGCTACA ACCCAGCAGGAAAGTTGC AD-AP1G2 Zm00001d036305 GCCATGGAGGCCAGTGAATTCAT GGACCTCGCCATCAACC ACGATTCATCTGCAGCTCGAGCTACAAC CCAGAAGGAAAGTTGC AD-RAD4 Zm00001d032245 GCCATGGAGGCCAGTGAATTCA TGCGGCGGACGAGGAGC ACGATTCATCTGCAGCTCGAGTTACAAC TCCTCTACTTGGATAGAAAAAC GST-CaM1 Zm00001d007194 GATCTGGTTCCGCGTGGATCCATGGCGG ACCAGCTCACC GTCACGATGCGGCCGCTCGAGTCA CTTGGCCATCATGACCTT qZmActin Actin TTGCTATCCAGGCTGTTCTT CGACCTCAGCAGCGCGGTCA