基于SSR技术的马铃薯种质资源遗传多样性分析

王丹, 张志成, 韩海霞, 韩娜, 王春勇, 王正, 潘孟洋, 李强, 王懿茜, 刘宇飞, 张丹

Genetic Diversity Analysis of Potato Germplasm Resources Based on SSR Technology

Wang Dan, Zhang Zhicheng, Han Haixia, Han Na, Wang Chunyong, Wang Zheng, Pan Mengyang, Li Qiang, Wang Yiqian, Liu Yufei, Zhang Dan

表2 SSR引物序列

Table 2 Sequence of SSR primers

引物名称Primer name 正向引物Forward primer(5’-3) 反向引物Reversed primer(5’-3’) C3 CCGGGAGAATTAGACGATGA TTTATGGGGAGCAGTTGAGG C32 CCCCTTACCCCTCTTCAATC AACCCGTGTCAACTTCTGCT C55 ACTGCTGTGGTTGGCGTC ACGGCATAGATTTGGAAGCATC C57 GACTGGCTGACCCTGAACTC GACAAAATTACAGGAACTGCAAA C59 TCATCACAACGTGACCCCA GGGCTTGAATGATGTGAAGCTC C61 CGCAAATCTTCATCCGATTC TCCGGCGGATAATACTTGTT S25 GCGAATGACAGGACAAGAGG TGCCACTGCTACCATAACCA S007 GACTGGCTGACCCTGAACTC GACAAAATTACAGGAACTGCAAA S118 AGAGATCGATGTAAAACACGT GTGGCATTTTGATGGATT S122 GACACGTTCACCATAAAA AGAAGAATAGCAAAGCAA S148 CAGCAAAATCAGAACCCGAT GGATCATCAAATTCACCGCT S151 GCTGCTAAACACTCAAGCAGAA CAACTACAAGATTCCATCCACAG S153 TATGTTCCACGCCATTTCAG ACGGAAACTCATCGTGCATT S168 CTGCCGCAAAAAGTGAAAAC TGAATGTAGGCCAAATTTTGAA S170 CGCAAATCTTCATCCGATTC TCCGGCGGATAATACTTGTT S174 TGAGGGTTTTCAGAAAGGGA CATCCTTGCAACAACCTCCT S184 TCATCACAACGTGACCCCA GGGCTTGAATGATGTGAAGCTC S189 CCTTGTAGTACAGCAGTGGTC TCCGCCAAGACTGATGCA S192 AATTCATGTTTGCGGTACGTC ATGCAGAAAGATGTCAAAATTGA STI005 CGCAAATCTTCATCCGATTC TCCGGCGGATAATACTTGTT STI027 ACTGCTGTGGTTGGCGTC ACGGCATAGATTTGGAAGCATC STI049 TCATCACAACGTGACCCC CGGGCTTGAATGATGTGAAGCT STACC ACAGGAATCACACCTGCACA TTCAACATCCGCCTGTCATA STGBSS AATCGGTGATAAATGTGAATGC ATGCTTGCCATGTGATGTGT STL001 CAGCAAATCAGAACCCGAT GGATCATCAAATTCACCGCT STL002 ACAGGAATCACACCTGCACA TTCAACATCCGCCTGTGATA STM0030 AGAGATCGATGTAAAACACGT GTGGCATTTTGATGGATT STM1031 GTGGTTTGTTTTTCTGTAT AATTCTATCCTCATCTCTA STM1058 ACAATTTAATTCAAGAAGCTAGG CCAAATTTGTATACTTCAATCTGA STM1064 GTTCTTTTGGTGGTTTTCCT TTATTTCTCTGTTGTTGCTG STM1104 TGATTCTCTTGCCTACTGTAATCG GAAAGTGGTGTGAAGCTGTGA STM2030 TCTTCCCAAATCTAGAATACATGC AAAGTTAGCATGGACAGCATTTC