基于SSR技术的马铃薯种质资源遗传多样性分析

王丹, 张志成, 韩海霞, 韩娜, 王春勇, 王正, 潘孟洋, 李强, 王懿茜, 刘宇飞, 张丹

Genetic Diversity Analysis of Potato Germplasm Resources Based on SSR Technology

Wang Dan, Zhang Zhicheng, Han Haixia, Han Na, Wang Chunyong, Wang Zheng, Pan Mengyang, Li Qiang, Wang Yiqian, Liu Yufei, Zhang Dan

表4 基于32对SSR引物的马铃薯材料遗传多样性分析

Table 4 Genetic diversity analysis of potato materials based on 32 pairs of SSR primers

引物 Primer 退火温度 Tm (℃) 多态性条带数 NBP 总条带数 Total bands 多态条带比率 PPB (%) 有效等位基因 Ne Nei’s遗传多样性指数 H Shannon指数 I 多态信息含量 PIC C3 56 3 4 75.00 1.6965 0.3607 0.5054 0.8108 C32 55 2 2 100.00 1.8985 0.4718 0.6645 0.6712 C55 55 3 3 100.00 1.7493 0.4229 0.6128 0.7951 C57 54 2 2 100.00 1.8438 0.4573 0.6498 0.5318 C59 53 3 4 75.00 1.5632 0.3200 0.4631 0.7484 C61 57 4 5 80.00 1.6947 0.3715 0.5256 0.8381 S25 55 7 9 77.78 1.7241 0.3744 0.5245 0.9198 S7 60 2 2 100.00 1.8331 0.4500 0.6413 0.6683 S118 58 8 10 80.00 1.5121 0.3275 0.4668 0.9248 S122 50 3 3 100.00 1.5146 0.3379 0.5202 0.6927 S148 53 3 4 75.00 1.5926 0.3307 0.4745 0.8231 S151 56 3 3 100.00 1.5545 0.3494 0.5310 0.7959 S153 53 7 8 87.50 1.8486 0.4306 0.5996 0.9085 S168 53 2 2 100.00 1.8504 0.4579 0.6501 0.4778 S170 53 7 8 87.50 1.7930 0.4144 0.5828 0.9142 S174 52 3 3 100.00 1.8569 0.4585 0.6503 0.7954 S184 53 4 5 80.00 1.7028 0.3730 0.5269 0.8500 S189 53 4 4 100.00 1.8574 0.4733 0.6661 0.8483 S192 54 6 7 85.71 1.6462 0.3635 0.5261 0.9084 STI005 56 6 7 85.71 1.7180 0.3878 0.5521 0.9033 STI027 55 5 6 83.33 1.5798 0.3330 0.4883 0.8766 STI049 53 3 3 100.00 1.9212 0.4790 0.6719 0.7895 STACC 55 4 4 100.00 1.4989 0.3322 0.5141 0.8521 STGBSS 54 3 3 100.00 1.4977 0.3304 0.5117 0.7962 STL001 56 6 7 85.71 1.6921 0.3778 0.5410 0.8971 STL002 55 3 4 75.00 1.6706 0.3527 0.4971 0.7754 STM0030 52 11 12 91.67 1.7709 0.4142 0.5893 0.9367 STM1031 50 5 5 100.00 1.7607 0.4217 0.6097 0.8660 STM1058 51 4 4 100.00 1.9674 0.4914 0.6845 0.8102 STM1064 56 6 7 85.71 1.6896 0.3702 0.5304 0.8880 STM1104 57 5 5 100.00 1.5269 0.3082 0.4562 0.8210 STM2030 55 8 9 88.89 1.8170 0.4234 0.5945 0.9262