du1新等位变异的克隆及其分子标记的开发

史亚兴, 刘俊玲, 朱贵川, 赫忠友, 刘辉, 樊艳丽, 徐丽, 卢柏山, 赵久然, 骆美洁

Cloning of the du1 Novel Allelic Variant and the Development of Its Molecular Markers

Shi Yaxing, Liu Junling, Zhu Guichuan, He Zhongyou, Liu Hui, Fan Yanli, Xu Li, Lu Baishan, Zhao Jiuran, Luo Meijie

表1 du1基因DNA扩增引物

Table 1 The primers for du1 gene DNA amplification

引物名称Primer name 5′→3′ DU3F CGTTTGCTGGGAAGTGGTTC DU3R AGTCTTGGCGCATAGTTGCT DU4F GCTCCAAGAAGAGCGAACAC DU4R ATAGCCAGCAACGGATTGGATT DU5F TCCAATCCGTTGCTGGCTAT DU5R GCCACAGCTGATAGATCACGA DU6F CGTGATCTATCAGCTGTGGC DU6R AGCAGGGCAAACGGTATTCT DU7F GACCATTTGGGCCATTCATCAC DU7R AGCACGTGAAAGACTAGTGAC DU8F GGCACCAATCGCAAAGGTTA DU8R GAGGGCTTCCCTTGCTGTATTT DU9F GCCTTTCCTATTTGGCAGCAC DU9R TTTTCCTTGTGTAGCCAGGCA DU10F GCGAAGTTGCACTGTTAGCTT DU10R TGTCAGGCGAGTGACGATTC DU11F GATGTCCCCGTCGTAGGAAT DU11R TACATTGGCCGTTGGTTGGAT DU12F CAACCAACGGCCAATGTAGT DU12R GGGTGAGAAGACAAAGATACCCT