du1新等位变异的克隆及其分子标记的开发

史亚兴, 刘俊玲, 朱贵川, 赫忠友, 刘辉, 樊艳丽, 徐丽, 卢柏山, 赵久然, 骆美洁

Cloning of the du1 Novel Allelic Variant and the Development of Its Molecular Markers

Shi Yaxing, Liu Junling, Zhu Guichuan, He Zhongyou, Liu Hui, Fan Yanli, Xu Li, Lu Baishan, Zhao Jiuran, Luo Meijie

表2 du1基因cDNA扩增及KASP分子标记的引物

Table 2 The primers for du1 gene cDNA amplification and KASP molecular marker assay

引物名称Primer name 5′→3′ cDNA扩增cDNA amplification DU3F CGTTTGCTGGGAAGTGGTTC DU4R ATAGCCAGCAACGGATTGGATT DX18F ATAGTTCACTTCCCTGAGCC DX6R TCAACGCTGCCATCATAGGT DX16F CAAGTCCCGTCATATGTGCT DX4R AGATGAATGCGGTGGTCGTAG DX10F CTACGACCACCGCATTCATCT DX2R TCAAGGTACCATCCTGGAGCT DX6F TTGAGGTTGAGTATCGTCCG DX1R GTAGTAGTCTGCCATTCTGGAC DUX5F CTGCACGACGTATTTCATGTGG DUX5R CCATTCTGTATGCCTGCTTAGG DU6F CGTGATCTATCAGCTGTGGC DU9R TTTTCCTTGTGTAGCCAGGCA DX17F GTCACTAGTCTTTCACGTGCTG DU11R TACATTGGCCGTTGGTTGGAT KASP分子标记KASP molecular marker AlleleX AAGAGAACCAACAATGGACTGGTG AlleleY AAGAGAACCAACAATGGACTGGTC Common1 TCCAATCCATCACCAGTTACGATAAACTA Common2 TCCGTGGTAGGGATCGTATTCCTAT