农杆菌介导的玉米叶片遗传转化体系的建立

盛奇明, 徐永盛, 荆风雪, 苏纯洁, 解光宁, 孙晓莎

Establishment of Agrobacterium-Mediated Genetic Transformation System for Maize Leaves

Sheng Qiming, Xu Yongsheng, Jing Fengxue, Su Chunjie, Xie Guangning, Sun Xiaosha

表2 不同玉米自交系的转化效率

Table 2 Transformation efficiency of different maize inbred lines

编号 Number 自交系 Inbred line 杂种优势群 Heterotic group 起始种子数 Number of starting seeds 碎叶片数 Number of chopped leaves 阳性苗数 Number of positive seedlings 转化效率 Transformation rate (%) 1 M013 Lancaster 11 1925 212 11.0 2 M018 Lancaster 10 1900 86 4.5 3 DH351 Reid 6 1092 36 3.3 4 F003 Reid 2 372 12 3.2 5 Qi319 PB 16 2752 52 1.9 6 PH4CV Lancaster 3 540 6 1.1