农杆菌碱基编辑技术的建立与recA基因精准编辑型菌株应用

孙梦琳, 符晓, 祁显涛, 刘昌林, 谢传晓, 郭晋杰, 朱金洁

Establishment of Base Editing Technology in Agrobacterium and Application of recA Gene Precisely Edited Strains

Sun Menglin, Fu Xiao, Qi Xiantao, Liu Changlin, Xie Chuanxiao, Guo Jinjie, Zhu Jinjie

表1 相关引物序列

Table 1 Related primer sequences

引物名称Primer name 引物序列（5’-3’）Primer sequence (5’-3’) Q26-HF1 CCTAGGTATAATACTAGTTCCCAGATCGAACGGTCGTTGTTTCAGAGCTATGCTGGAAA Q26-HF2 TCCTCTAGAGTCGACCTGCAGGTTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTTC Q26-HF3 TTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGG ScaffT2T-HR TTAAAAAAAGGCCATCCGTCAGGATGGCCTTCTGCACCGACTCGGTGCCACTTTTTCA Lac-flag-HF1 CTTTATGCTTCCGGCTCGTATGTTAGGAGGTCTTTATCATGGACTATAAGGACCACGAC Lac-flag-HF2 CCATCCTGACGGATGGCCTTTTTTTAAGCTTTTTACACTTTATGCTTCCGGCTCGTATG Flag-CDA1-HR GAATCCGCACGTACTCGGCGTCGGTCTTATCGTCATCGTCTTTGTAA Flag-CDA1-HF GATGACGATAAGACCGACGCCGAGTACGTGCGGATTCAC CDA1-cas9-HR GAGGCCGATGGAGTACTTCTTGTCCACGGCGGGAGACTTTGTGGTGTGC Cas9-HF GACAAGAAGTACTCCATCGGCCTCGCCAT UGI-T3T-HR1 GAAAGAAGAAAGGGGCAATAACGCCCCACATTTTATACGAGCATCTTAATCTTGTTCTC T3T-MaubI-HR2 ATTCCCGATCTAGTAACATAGATGACACCGCGCGCGAGAAAGAAGAAAGGGGCAATAAC T2-virB-HF CCATCCTGACGGATGGCCTTTTTTTAAGCTTCGAGCATTTTCGTTCTGAGCCTGACCTG VirB-flag-HR GTCGTGGTCCTTATAGTCCATACCTTATCTCCTTAGCTCGCAACTA VirB-flag-HF AGTTGCGAGCTAAGGAGATAAGGTATGGACTATAAGGACCACGA CDA1-cas9-HR GAGGCCGATGGAGTACTTCTTGTCCACGGCGGGAGACTTTGTGGTGTGC Lac-flag-HF1 CTTTATGCTTCCGGCTCGTATGTTAGGAGGTCTTTATCATGGACTATAAGGACCACGAC Lac-flag-HF2 CCATCCTGACGGATGGCCTTTTTTTAAGCTTTTTACACTTTATGCTTCCGGCTCGTATG Flag-T8e-HR ACTCGTGGGAAAACTCCACCTCAGACTTATCGTCATCGTCTTTGTAA TadA8e-HF ACTCGTGGGAAAACTCCACCTCAGACTTATCGTCATCGTCTTTGTAA Cas9-T3T-HR AAAGAAGAAAGGGGCAATAACGCCCCACATTTTATACGTCGCCGCCGAGCTGGGAGAGG TadA8e-HF TCTGAGGTGGAGTTTTCCCACGAGT TadA-cas9-HR GAGGCCGATGGAGTACTTCTTGTCGTCTGTGGAGCTCTGGGCCTTCTTCTGA JCF-3301 CACTCATTAGGCACCCCAGG JCR-Cas9 AAAATCTCCTGGAGGTAGCAG JCF-H840A AAGAAGGGCATCCTCCAGAC JCR-3301 CTTTGATCCCGAGGGGAACC JCF-Q26 CAAATCCACACCGAGCTTGC JCR-Q26 GGCACGGAAAAACGAAAGCA