农杆菌碱基编辑技术的建立与recA基因精准编辑型菌株应用

孙梦琳, 符晓, 祁显涛, 刘昌林, 谢传晓, 郭晋杰, 朱金洁

Establishment of Base Editing Technology in Agrobacterium and Application of recA Gene Precisely Edited Strains

Sun Menglin, Fu Xiao, Qi Xiantao, Liu Changlin, Xie Chuanxiao, Guo Jinjie, Zhu Jinjie

表2 不同腺嘌呤脱氨酶变体

Table 2 Different adenine deaminase variants

变体 Variant 位点Site 23 26 27 28 36 47 48 51 61 73 76 82 84 96 106 108 109 110 111 TadAWT W R E V H R P R M Y I V L H A D A K T TadA8e R L A L F W N TadA8.20 R L A L Y S F W N TadA8r R L K A L Y S W G R H 变体 Variant 位点Site 114 119 122 123 126 127 146 147 149 152 154 155 156 157 158 161 166 167 TadAWT A D H H M N S D F R Q E I K A K T D TadA8e Y C Y P V F N TadA8.20 C R P R V F N TadA8r V N Y I K C R P R V F