农杆菌碱基编辑技术的建立与recA基因精准编辑型菌株应用

孙梦琳, 符晓, 祁显涛, 刘昌林, 谢传晓, 郭晋杰, 朱金洁

Establishment of Base Editing Technology in Agrobacterium and Application of recA Gene Precisely Edited Strains

Sun Menglin, Fu Xiao, Qi Xiantao, Liu Changlin, Xie Chuanxiao, Guo Jinjie, Zhu Jinjie

图3 EHA105（ΔrecA）突变型菌株的DNA损伤测试 (a) 卡那霉素敏感性测试；(b) 载体特异性引物扩增PCR；(c) recA突变位点Sanger测序验证；(d) DNA损伤（0.005%MMS）测试。

Fig.3 DNA damage testing of EHA105(ΔrecA) mutant strains (a) Kanamycin sensitivity recovery test (b) PCR amplification of specific primers after eliminating the editing vector (c) Sanger sequencing verification of recA mutation sites (d) DNA damage (0.005% MMS) test.