农杆菌碱基编辑技术的建立与recA基因精准编辑型菌株应用

孙梦琳, 符晓, 祁显涛, 刘昌林, 谢传晓, 郭晋杰, 朱金洁

Establishment of Base Editing Technology in Agrobacterium and Application of recA Gene Precisely Edited Strains

Sun Menglin, Fu Xiao, Qi Xiantao, Liu Changlin, Xie Chuanxiao, Guo Jinjie, Zhu Jinjie

图4 基于EHA105（ΔrecA）菌株的三元载体系统对玉米幼胚的瞬时侵染 (a) pVS1或pRiA4复制子辅助质粒载体结构示意图；(b) 表达报告基因的双元载体p3301-GUS和p3301-CRC的结构示意图；(c) 基于EHA105（ΔrecA）菌株的三元载体系统对玉米幼胚的瞬时侵染；(d) 基于EHA105(ΔrecA)菌株的三元系统侵染效率（n=18）；“****”表示差异极显著（P < 0.0001）。

Fig.4 Transient infection of maize immature embryos using the ternary vector system based on the EHA105(ΔrecA) strain (a) schematic diagram of helper plasmid vector structures; (b) schematic diagram of reporter binary vectors; (c) transient infection of maize immature embryos using the ternary vector system based on the EHA105(ΔrecA) strain; (d) ternary systemic infection efficiency based on EHA105(ΔrecA) strain (n=18),“****”indicates extremely significant differences (P < 0.0001).