利用CRISPR/Cas9技术编辑GS3、GS9和GW2基因改良水稻粒型

阙涛, 吴娴, 王忠妮, 吴钱蓉, 龙武华, 王倩, 朱速松

Improvement of Rice Grain Shape by Editing of GS3, GS9 and GW2 Genes Using CRISPR/Cas9 Technology

Que Tao, Wu Xian, Wang Zhongni, Wu Qianrong, Long Wuhua, Wang Qian, Zhu Susong

表1 引物信息

Table 1 Information of primers

引物名称Primer name 引物序列（5′-3′）Primer sequence (5′-3′) Cas-9-gRT1 ACGCGCTCCACCGCGAGATgttttagagctagaaat Cas-9-OsU6aT1-R ATCTCGCGGTGGAGCGCGTCggcagccaagccagca Cas-9-gRT2 CGATTGCTTCCTGCTCGGTTgttttagagctagaaat Cas-9-OsU6bT2-R AACCGAGCAGGAAGCAATCGCaacacaagcggcagc Cas-9-gRT3 AAGCTCGCGCCGTGCTACATgttttagagctagaaat Cas-9-OsU3T3-R ATGTAGCACGGCGCGAGCTTCtgagcctcagcgcag U-F CTCCGTTTTACCTGTGGAATCG gRNA-R CGGAGGAAAATTCCATCCAC Pps-R TTCAGAggtctcT accg ACTAGTATGGAATCGGCAGCAAAGG Pgs-L AGCGTGggtctcG ctcg ACGCGTATCCATCCACTCCAAGCTC SP-L GCGGTGTCATCTATGTTACTAG RB-R AAGTTGGGTAACGCCAGGGT Cas9-F GAGACTATCACCCCTTGGAA Cas9-R GATGAGCGTAAGTCTTGAGC GS3-F CGGAGTGACATGGCAATGG GS3-R TTCGACAGATAGCAAGCCGT GS9-F AGCTGCAGGGAGTGTCCT GS9-R AGCAGGGCACGTACAGAGT GW2-F GAGTGGTGAGGGTTTCATCTG GW2-R TACCAGGAAGCAGATGGGG