利用CRISPR/Cas9技术编辑GS3、GS9和GW2基因改良水稻粒型

阙涛, 吴娴, 王忠妮, 吴钱蓉, 龙武华, 王倩, 朱速松

Improvement of Rice Grain Shape by Editing of GS3, GS9 and GW2 Genes Using CRISPR/Cas9 Technology

Que Tao, Wu Xian, Wang Zhongni, Wu Qianrong, Long Wuhua, Wang Qian, Zhu Susong

图2 载体图谱 插入片段区域包含35S启动子激活的潮霉素磷酸转移酶基因，泛素启动子激活的Cas9基因，U6a、U6b和U3启动子激活的具有敲除靶点的GS3/GS9/GW2-sgRNA表达盒；LB：T-DNA左边界；RB：T-DNA右边界。

Fig.2 Vector diagram The insert region contains the hygromycin phosphotransferase gene activated by the 35S promoter, the Cas9 gene activated by the ubiquitin promoter, and the GS3/GS9/GW2-sgRNA expression cassette with knockout targets activated by the U6a, U6b, and U3 promoters; LB: left border of T-DNA; RB: T-DNA right border.